High-level aminoglycoside resistance and virulence characteristics among Enterococci isolated from recreational beaches in Malaysia

Ayokunle Christopher Dada, Asmat Ahmad, Gires Usup, Yook Heng Lee, Rahimi Hamid

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We report the first study on the occurrence of high-level aminoglycoside-resistant (HLAR) Enterococci in coastal bathing waters and beach sand in Malaysia. None of the encountered isolates were resistant to high levels of gentamicin (500 μg/mL). However, high-level resistance to kanamycin (2,000 μg/mL) was observed in 14.2 % of tested isolates, the highest proportions observed being among beach sand isolates. High-level resistance to kanamycin was higher among Enterococcus faecalis and Enterococcus faecium than Enterococcus spp. Chi-square analysis showed no significant association between responses to tested antibiotics and the species allocation or source of isolation of all tested Enterococci. The species classification of encountered Enterococci resistance to vancomycin was highest among Enterococcus spp. (5.89 %) followed by E. faecium (4.785) and least among E. faecalis. A total of 160 isolates were also tested for virulence characteristics. On the whole, caseinase production was profoundly highest (15.01 %) while the least prevalent virulence characteristic observed among tested beach Enterococci was haemolysis of rabbit blood (3.65 %). A strong association was observed between the source of isolation and responses for each of caseinase (C = 0.47, V = 0.53) and slime (C = 0.50, V = 0.58) assays. Analysis of obtained spearman's coefficient showed a strong correlation between caseinase and each of the slime production (p = 0.04), gelatinase (p = 0.0035) and haemolytic activity on horse blood (p = 0.004), respectively. Suggestively, these are the main virulent characteristics of the studied beach Enterococci. Our findings suggest that recreational beaches may contribute to the dissemination of Enterococci with HLAR and virulence characteristics.

Original languageEnglish
Pages (from-to)7427-7443
Number of pages17
JournalEnvironmental Monitoring and Assessment
Volume185
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Beaches
virulence
beach
Blood
Sand
blood
Association reactions
bathing water
sand
Antibiotics
horse
antibiotics
Assays
Aminoglycosides
assay
Water
analysis

Keywords

  • Beach sand
  • Enterococcus
  • High-level aminoglycoside resistance
  • Malaysia
  • Recreational beach

ASJC Scopus subject areas

  • Environmental Science(all)
  • Management, Monitoring, Policy and Law
  • Pollution

Cite this

High-level aminoglycoside resistance and virulence characteristics among Enterococci isolated from recreational beaches in Malaysia. / Dada, Ayokunle Christopher; Ahmad, Asmat; Usup, Gires; Lee, Yook Heng; Hamid, Rahimi.

In: Environmental Monitoring and Assessment, Vol. 185, No. 9, 09.2013, p. 7427-7443.

Research output: Contribution to journalArticle

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