High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multilocus sequence typing from a hospital in Malaysia

Poh Leng Weng, Ramliza Ramli, Mariana Nor Shamsudin, Yoke Kqueen Cheah, Rukman Awang Hamat

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29 Citations (Scopus)

Abstract

Little is known on the genetic relatedness and potential dissemination of particular enterococcal clones in Malaysia. We studied the antibiotic susceptibility profiles of Enterococcus faecium and Enterococcus faecalis and subjected them to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). E. faecium and E. faecalis displayed 27 and 30 pulsotypes, respectively, and 10 representative E. faecium and E. faecalis isolates (five each) yielded few different sequence types (STs): ST17 (2 isolates), ST78, ST203, and ST601 for E. faecium, and ST6, ST16, ST28, ST179, and ST399 for E. faecalis. Resistance to tazobactam-piperacillin and ampicillin amongst E. faecium isolates was highly observed as compared to E. faecalis isolates. All of the isolates were sensitive to vancomycin and teicoplanin. The presence of epidemic and nosocomial strains of selected E. faecium STs: 17, 78, and 203 and E. faecalis ST6 as well as high rates of resistance to multiple antibiotics amongst E. faecium isolates is of a particular concern.

Original languageEnglish
Article number938937
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013

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Multilocus Sequence Typing
Enterococcus faecium
Enterococcus faecalis
Pulsed Field Gel Electrophoresis
Malaysia
Electrophoresis
Gels
Anti-Bacterial Agents
Teicoplanin
Vancomycin
Ampicillin
Clone Cells
interleukin-24
tazobactam drug combination piperacillin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

High genetic diversity of Enterococcus faecium and Enterococcus faecalis clinical isolates by pulsed-field gel electrophoresis and multilocus sequence typing from a hospital in Malaysia. / Weng, Poh Leng; Ramli, Ramliza; Shamsudin, Mariana Nor; Cheah, Yoke Kqueen; Hamat, Rukman Awang.

In: BioMed Research International, Vol. 2013, 938937, 2013.

Research output: Contribution to journalArticle

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