Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae

Zhongshu Song, Walid Bakeer, James W. Marshall, Ahmed A. Yakasai, Rozida Mohd. Khalid, Jerome Collemare, Elizabeth Skellam, Didier Tharreau, Marc Henri Lebrun, Colin M. Lazarus, Andrew M. Bailey, Thomas J. Simpson, Russell J. Cox

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.

Original languageEnglish
Pages (from-to)4837-4845
Number of pages9
JournalChemical Science
Volume6
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015
Externally publishedYes

Fingerprint

Pathogens
Introns
Genes
Amides
Pyrones
Aspergillus
Cluster analysis
Fungi
Tyrosine
Testing

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Song, Z., Bakeer, W., Marshall, J. W., Yakasai, A. A., Mohd. Khalid, R., Collemare, J., ... Cox, R. J. (2015). Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. Chemical Science, 6(8), 4837-4845. https://doi.org/10.1039/c4sc03707c

Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. / Song, Zhongshu; Bakeer, Walid; Marshall, James W.; Yakasai, Ahmed A.; Mohd. Khalid, Rozida; Collemare, Jerome; Skellam, Elizabeth; Tharreau, Didier; Lebrun, Marc Henri; Lazarus, Colin M.; Bailey, Andrew M.; Simpson, Thomas J.; Cox, Russell J.

In: Chemical Science, Vol. 6, No. 8, 01.08.2015, p. 4837-4845.

Research output: Contribution to journalArticle

Song, Z, Bakeer, W, Marshall, JW, Yakasai, AA, Mohd. Khalid, R, Collemare, J, Skellam, E, Tharreau, D, Lebrun, MH, Lazarus, CM, Bailey, AM, Simpson, TJ & Cox, RJ 2015, 'Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae', Chemical Science, vol. 6, no. 8, pp. 4837-4845. https://doi.org/10.1039/c4sc03707c
Song, Zhongshu ; Bakeer, Walid ; Marshall, James W. ; Yakasai, Ahmed A. ; Mohd. Khalid, Rozida ; Collemare, Jerome ; Skellam, Elizabeth ; Tharreau, Didier ; Lebrun, Marc Henri ; Lazarus, Colin M. ; Bailey, Andrew M. ; Simpson, Thomas J. ; Cox, Russell J. / Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. In: Chemical Science. 2015 ; Vol. 6, No. 8. pp. 4837-4845.
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