Hepatoprotective effects of zerumbone against paracetamol-induced acute hepatotoxicity in rats

Asmah Hamid, Liow Say Lee, Saiful Ridzuan Karim, Nurul Farhana Jufri

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Zerumbone (ZER) is a major bioactive compound of Zingiber zerumbet, a wild ginger plant that has been documented to have anti-proliferative, anti-inflammatory and anti-oxidant properties. To investigate its hepatoprotective potential, this study was designed to determine the treatment effects of ZER on acute hepatotoxicity induced by paracetamol (PCM) in rats. Methods: The control group was administered with phosphate buffer solution (PBS) while the other two groups received PCM alone (1000 mg/kg) and PCM + 25 mg/kg ZER, respectively, at 0 h and 4 h after PCM injection. After 24 h, the blood and liver were collected for differential white blood cell count, liver histological observation and biochemical analysis including alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total protein concentration in serum and liver. Results: Treatment with ZER was found to significantly reduce ALT (P = 0.041), AST (P = 0.044) and total hepatic protein (P = 0.045) in comparison to PCM-induced rats. Rats treated with ZER exhibited the normal structure of hepatocytes with no vacuolisation or necrosis and showed significantly reduced neutrophil count (P = 0.037). This finding suggests its ability to suppress the inflammatory processes caused by PCM overdosage and decrease the hepatocytes tendency to go through necrotic processes. Conclusion: ZER possessed protective activity against PCM-induced acute hepatotoxicity in a rat model.

Original languageEnglish
Pages (from-to)64-71
Number of pages8
JournalMalaysian Journal of Medical Sciences
Volume25
Issue number2
DOIs
Publication statusPublished - 1 Apr 2018

Fingerprint

Acetaminophen
Liver
Aspartate Aminotransferases
Alanine Transaminase
Asarum
Hepatocytes
zerumbone
Leukocyte Count
Oxidants
Buffers
Proteins
Neutrophils
Anti-Inflammatory Agents
Necrosis
Phosphates
Observation
Control Groups
Injections
Therapeutics
Serum

Keywords

  • Liver
  • Oxidative stress
  • Paracetamol
  • Toxicity
  • Zerumbone

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hepatoprotective effects of zerumbone against paracetamol-induced acute hepatotoxicity in rats. / Hamid, Asmah; Lee, Liow Say; Karim, Saiful Ridzuan; Jufri, Nurul Farhana.

In: Malaysian Journal of Medical Sciences, Vol. 25, No. 2, 01.04.2018, p. 64-71.

Research output: Contribution to journalArticle

Hamid, Asmah ; Lee, Liow Say ; Karim, Saiful Ridzuan ; Jufri, Nurul Farhana. / Hepatoprotective effects of zerumbone against paracetamol-induced acute hepatotoxicity in rats. In: Malaysian Journal of Medical Sciences. 2018 ; Vol. 25, No. 2. pp. 64-71.
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