Hepatoprotective effect of Cymbopogon citratus aqueous extract against hydrogen peroxide-induced liver injury in male rats

Saleh M uhammad Rahim, Ekhlass M uhi Taha, Muneef S aeb Al-janabi, Bushra I smael Al-douri, Simon Kumar Das, Mazlan Abd. Ghaffar

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

BACKGROUND: Cymbopogon citratus (Poaceae) a tropical perennial herb plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. This study aimed at evaluating the protective effects of C. citratus aqueous extract against liver injury induced by hydrogen peroxide (H2O2), in male rats.

MATERIALS AND METHODS: Twenty-five rats were randomly divided into five different groups of five animals in each group; (1) Control. (2) Received H2O2 (0.5%) with drinking water. (3), and (4) received H2O2 and C. citratus (100 mg·kg(-1) b wt), vitamin C (250 mg·kg(-1) b wt) respectively. (5), was given C. citratus alone. The treatments were administered for 30 days. Blood samples were collected and serum was used for biochemical assay including liver enzymes activities, total protein, total bilirubin and malonaldehyde, glutathione in serum and liver homogenates. Liver was excised and routinely processed for histological examinations.

RESULTS: C. citratus attenuated liver damage due to H2O2 administration as indicated by the significant reduction (p<0.05), in the elevated levels of ALT, AST, ALP, LDH, TB, and MDA in serum and liver homogenates; increase in TP and GSH levels in serum and liver homogenates; and improvement of liver histo-pathological changes. These effects of the extract were similar to that of vitamin C which used as antioxidant reference.

CONCLUSION: C. citratus could effectively ameliorate H2O2-induced oxidative stress and prevent liver injury in male rats.

Original languageEnglish
Pages (from-to)447-451
Number of pages5
JournalAfrican Journal of Traditional, Complementary and Alternative Medicines
Volume11
Issue number2
Publication statusPublished - 2014

Fingerprint

Cymbopogon
Hydrogen Peroxide
Liver
Wounds and Injuries
Serum
Ascorbic Acid
Preserved Food
Carbonated Beverages
Liver Extracts
Tea
Poaceae
Malondialdehyde
Bilirubin
Drinking Water
Glutathione
Oxidative Stress
Antioxidants

Keywords

  • Cymbopogon citratus
  • Histopathology
  • Hydrogen peroxide
  • Liver enzymes
  • Rat
  • Serum proteins
  • Vitamin C

ASJC Scopus subject areas

  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Hepatoprotective effect of Cymbopogon citratus aqueous extract against hydrogen peroxide-induced liver injury in male rats. / Rahim, Saleh M uhammad; Taha, Ekhlass M uhi; Al-janabi, Muneef S aeb; Al-douri, Bushra I smael; Das, Simon Kumar; Abd. Ghaffar, Mazlan.

In: African Journal of Traditional, Complementary and Alternative Medicines, Vol. 11, No. 2, 2014, p. 447-451.

Research output: Contribution to journalArticle

@article{bd695f87b743447bb0c7355420e28b33,
title = "Hepatoprotective effect of Cymbopogon citratus aqueous extract against hydrogen peroxide-induced liver injury in male rats",
abstract = "BACKGROUND: Cymbopogon citratus (Poaceae) a tropical perennial herb plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. This study aimed at evaluating the protective effects of C. citratus aqueous extract against liver injury induced by hydrogen peroxide (H2O2), in male rats.MATERIALS AND METHODS: Twenty-five rats were randomly divided into five different groups of five animals in each group; (1) Control. (2) Received H2O2 (0.5{\%}) with drinking water. (3), and (4) received H2O2 and C. citratus (100 mg·kg(-1) b wt), vitamin C (250 mg·kg(-1) b wt) respectively. (5), was given C. citratus alone. The treatments were administered for 30 days. Blood samples were collected and serum was used for biochemical assay including liver enzymes activities, total protein, total bilirubin and malonaldehyde, glutathione in serum and liver homogenates. Liver was excised and routinely processed for histological examinations.RESULTS: C. citratus attenuated liver damage due to H2O2 administration as indicated by the significant reduction (p<0.05), in the elevated levels of ALT, AST, ALP, LDH, TB, and MDA in serum and liver homogenates; increase in TP and GSH levels in serum and liver homogenates; and improvement of liver histo-pathological changes. These effects of the extract were similar to that of vitamin C which used as antioxidant reference.CONCLUSION: C. citratus could effectively ameliorate H2O2-induced oxidative stress and prevent liver injury in male rats.",
keywords = "Cymbopogon citratus, Histopathology, Hydrogen peroxide, Liver enzymes, Rat, Serum proteins, Vitamin C",
author = "Rahim, {Saleh M uhammad} and Taha, {Ekhlass M uhi} and Al-janabi, {Muneef S aeb} and Al-douri, {Bushra I smael} and Das, {Simon Kumar} and {Abd. Ghaffar}, Mazlan",
year = "2014",
language = "English",
volume = "11",
pages = "447--451",
journal = "African Journal of Traditional, Complementary and Alternative Medicines",
issn = "0189-6016",
publisher = "African Ethnomedicines Network",
number = "2",

}

TY - JOUR

T1 - Hepatoprotective effect of Cymbopogon citratus aqueous extract against hydrogen peroxide-induced liver injury in male rats

AU - Rahim, Saleh M uhammad

AU - Taha, Ekhlass M uhi

AU - Al-janabi, Muneef S aeb

AU - Al-douri, Bushra I smael

AU - Das, Simon Kumar

AU - Abd. Ghaffar, Mazlan

PY - 2014

Y1 - 2014

N2 - BACKGROUND: Cymbopogon citratus (Poaceae) a tropical perennial herb plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. This study aimed at evaluating the protective effects of C. citratus aqueous extract against liver injury induced by hydrogen peroxide (H2O2), in male rats.MATERIALS AND METHODS: Twenty-five rats were randomly divided into five different groups of five animals in each group; (1) Control. (2) Received H2O2 (0.5%) with drinking water. (3), and (4) received H2O2 and C. citratus (100 mg·kg(-1) b wt), vitamin C (250 mg·kg(-1) b wt) respectively. (5), was given C. citratus alone. The treatments were administered for 30 days. Blood samples were collected and serum was used for biochemical assay including liver enzymes activities, total protein, total bilirubin and malonaldehyde, glutathione in serum and liver homogenates. Liver was excised and routinely processed for histological examinations.RESULTS: C. citratus attenuated liver damage due to H2O2 administration as indicated by the significant reduction (p<0.05), in the elevated levels of ALT, AST, ALP, LDH, TB, and MDA in serum and liver homogenates; increase in TP and GSH levels in serum and liver homogenates; and improvement of liver histo-pathological changes. These effects of the extract were similar to that of vitamin C which used as antioxidant reference.CONCLUSION: C. citratus could effectively ameliorate H2O2-induced oxidative stress and prevent liver injury in male rats.

AB - BACKGROUND: Cymbopogon citratus (Poaceae) a tropical perennial herb plant that is widely cultivated to be eaten either fresh with food or dried in tea or soft drink has been reported to possess a number of medicinal and aromatic properties. This study aimed at evaluating the protective effects of C. citratus aqueous extract against liver injury induced by hydrogen peroxide (H2O2), in male rats.MATERIALS AND METHODS: Twenty-five rats were randomly divided into five different groups of five animals in each group; (1) Control. (2) Received H2O2 (0.5%) with drinking water. (3), and (4) received H2O2 and C. citratus (100 mg·kg(-1) b wt), vitamin C (250 mg·kg(-1) b wt) respectively. (5), was given C. citratus alone. The treatments were administered for 30 days. Blood samples were collected and serum was used for biochemical assay including liver enzymes activities, total protein, total bilirubin and malonaldehyde, glutathione in serum and liver homogenates. Liver was excised and routinely processed for histological examinations.RESULTS: C. citratus attenuated liver damage due to H2O2 administration as indicated by the significant reduction (p<0.05), in the elevated levels of ALT, AST, ALP, LDH, TB, and MDA in serum and liver homogenates; increase in TP and GSH levels in serum and liver homogenates; and improvement of liver histo-pathological changes. These effects of the extract were similar to that of vitamin C which used as antioxidant reference.CONCLUSION: C. citratus could effectively ameliorate H2O2-induced oxidative stress and prevent liver injury in male rats.

KW - Cymbopogon citratus

KW - Histopathology

KW - Hydrogen peroxide

KW - Liver enzymes

KW - Rat

KW - Serum proteins

KW - Vitamin C

UR - http://www.scopus.com/inward/record.url?scp=84990879171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990879171&partnerID=8YFLogxK

M3 - Article

VL - 11

SP - 447

EP - 451

JO - African Journal of Traditional, Complementary and Alternative Medicines

JF - African Journal of Traditional, Complementary and Alternative Medicines

SN - 0189-6016

IS - 2

ER -