Hepatic metallothionein and Glutathione-S-Transferase responses in two populations of rice frogs, Fejervarya limnocharis, naturally exposed to different environmental cadmium levels

Mohd. Sham Othman, Wichase Khonsue, Jirarach Kitana, Kumthorn Thirakhupt, Mark Robson, Marija Borjan, Noppadon Kitana

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glutathione-S-Transferase (GST) and metallothionein are important biomarker endpoints in studying the effect of Cd exposure. The purpose of this research was to study the correlation between hepatic GST and metallothionein with hepatic Cd in wild Fejervarya limnocharis exposed to environmental Cd. Results showed that frogs from contaminated sites had significantly higher hepatic metallothionein (3.58 mg/kg wet weight) and GST activity (0.259 μmol/min/mg total protein) than those from the reference site (2.36 mg/kg wet weight and 0.157 μmol/ min/mg total protein respectively). There was a significantly positive correlation between hepatic Cd and GST activity (r = 0.802, p = 0.009) but not between hepatic Cd and metallothionein (r = 0.548, p = 0.139). The results concluded that while frogs from the contaminated site had higher GST and metallothionein, only GST showed significant positive correlation with hepatic Cd levels, indicating that hepatic GST activity may be used as a biomarker endpoint.

Original languageEnglish
Pages (from-to)225-228
Number of pages4
JournalBulletin of Environmental Contamination and Toxicology
Volume89
Issue number2
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Metallothionein
Glutathione Transferase
Cadmium
Anura
Liver
Population
Biomarkers
Proteins
Weights and Measures
Oryza
Glutathione
Research

Keywords

  • Biomarker
  • Cd
  • Fejervarya limnocharis
  • GST
  • Metallothionein

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Toxicology

Cite this

Hepatic metallothionein and Glutathione-S-Transferase responses in two populations of rice frogs, Fejervarya limnocharis, naturally exposed to different environmental cadmium levels. / Othman, Mohd. Sham; Khonsue, Wichase; Kitana, Jirarach; Thirakhupt, Kumthorn; Robson, Mark; Borjan, Marija; Kitana, Noppadon.

In: Bulletin of Environmental Contamination and Toxicology, Vol. 89, No. 2, 08.2012, p. 225-228.

Research output: Contribution to journalArticle

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