HbA2 levels in normal, β-thalassaemia and haemoglobin E carriers by capillary electrophoresis

Hafiza Alauddin, Mohd Yusoff Malisa, R. D AidifiTrina Khirotdin, Azlin Ithnin, Raja Zahratul Azma Raja Sabudin, Matthew Chong Kwok Thong, Irwan Mohamad Ali, Zi Ning Yeoh, Lailyvia Mohd Ishak, Nur Rabiatuladawiah Mohd Radzi, Noor Hamidah Hussin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: The capillary electrophoresis (CE) is a new system that utilizes the principle of electrokinetic separation of molecules in eight electrolyte buffer-filled silica capillaries. In this study, we established the normal ranges of haemoglobin A2 (HbA2) and haemoglobin F (HbF) levels for normal individuals using this system and also the HbA2 level in β thalassaemia and haemoglobin E (HbE) individuals. Materials and Methods: 154 samples from normal individuals, 218 samples from β thalassaemia heterozygotes and 91 samples from HbE heterozygotes were subjected to high performance liquid chromatography (HPLC) and CE analysis. Results: The normal ranges for HbA2 and HbF by CE were 2.75% (SD 0.26%) and 0.03% (SD 0.24%) respectively, which were significantly lower than that of HPLC 2.88% (SD 0.25%) and 0.58% (SD 0.61%) (p <0.001). The HbA2 level for HbE heterozygotes was 3.58% (SD 0.44%), which was significantly higher than normal (p <0.001) but lower than that of β-thalassaemia heterozygotes (p<0.001) and the true HbE level was 24.28% (SD 3.38%). Conclusion: The CE system provided a fully automated and high throughput system for haemoglobin analysis. We established the normal ranges for HbA2 and HbF levels by CE. We also determined the ranges for HbA2 in beta thalassaemia and HbE heterozygotes using this system.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalMalaysian Journal of Pathology
Volume34
Issue number2
Publication statusPublished - Dec 2012

Fingerprint

Hemoglobin A2
Hemoglobin E
Thalassemia
Capillary Electrophoresis
Heterozygote
Fetal Hemoglobin
Reference Values
High Pressure Liquid Chromatography
beta-Thalassemia
Silicon Dioxide
Electrolytes
Buffers
Hemoglobins

Keywords

  • βthalassaemia
  • Capillary electrophoresis
  • Haemoglobin E
  • HbA
  • Normal range

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

HbA2 levels in normal, β-thalassaemia and haemoglobin E carriers by capillary electrophoresis. / Alauddin, Hafiza; Malisa, Mohd Yusoff; Khirotdin, R. D AidifiTrina; Ithnin, Azlin; Raja Sabudin, Raja Zahratul Azma; Thong, Matthew Chong Kwok; Mohamad Ali, Irwan; Yeoh, Zi Ning; Mohd Ishak, Lailyvia; Mohd Radzi, Nur Rabiatuladawiah; Hussin, Noor Hamidah.

In: Malaysian Journal of Pathology, Vol. 34, No. 2, 12.2012, p. 161-164.

Research output: Contribution to journalArticle

Alauddin, H, Malisa, MY, Khirotdin, RDA, Ithnin, A, Raja Sabudin, RZA, Thong, MCK, Mohamad Ali, I, Yeoh, ZN, Mohd Ishak, L, Mohd Radzi, NR & Hussin, NH 2012, 'HbA2 levels in normal, β-thalassaemia and haemoglobin E carriers by capillary electrophoresis', Malaysian Journal of Pathology, vol. 34, no. 2, pp. 161-164.
Alauddin, Hafiza ; Malisa, Mohd Yusoff ; Khirotdin, R. D AidifiTrina ; Ithnin, Azlin ; Raja Sabudin, Raja Zahratul Azma ; Thong, Matthew Chong Kwok ; Mohamad Ali, Irwan ; Yeoh, Zi Ning ; Mohd Ishak, Lailyvia ; Mohd Radzi, Nur Rabiatuladawiah ; Hussin, Noor Hamidah. / HbA2 levels in normal, β-thalassaemia and haemoglobin E carriers by capillary electrophoresis. In: Malaysian Journal of Pathology. 2012 ; Vol. 34, No. 2. pp. 161-164.
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abstract = "Objective: The capillary electrophoresis (CE) is a new system that utilizes the principle of electrokinetic separation of molecules in eight electrolyte buffer-filled silica capillaries. In this study, we established the normal ranges of haemoglobin A2 (HbA2) and haemoglobin F (HbF) levels for normal individuals using this system and also the HbA2 level in β thalassaemia and haemoglobin E (HbE) individuals. Materials and Methods: 154 samples from normal individuals, 218 samples from β thalassaemia heterozygotes and 91 samples from HbE heterozygotes were subjected to high performance liquid chromatography (HPLC) and CE analysis. Results: The normal ranges for HbA2 and HbF by CE were 2.75{\%} (SD 0.26{\%}) and 0.03{\%} (SD 0.24{\%}) respectively, which were significantly lower than that of HPLC 2.88{\%} (SD 0.25{\%}) and 0.58{\%} (SD 0.61{\%}) (p <0.001). The HbA2 level for HbE heterozygotes was 3.58{\%} (SD 0.44{\%}), which was significantly higher than normal (p <0.001) but lower than that of β-thalassaemia heterozygotes (p<0.001) and the true HbE level was 24.28{\%} (SD 3.38{\%}). Conclusion: The CE system provided a fully automated and high throughput system for haemoglobin analysis. We established the normal ranges for HbA2 and HbF levels by CE. We also determined the ranges for HbA2 in beta thalassaemia and HbE heterozygotes using this system.",
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T1 - HbA2 levels in normal, β-thalassaemia and haemoglobin E carriers by capillary electrophoresis

AU - Alauddin, Hafiza

AU - Malisa, Mohd Yusoff

AU - Khirotdin, R. D AidifiTrina

AU - Ithnin, Azlin

AU - Raja Sabudin, Raja Zahratul Azma

AU - Thong, Matthew Chong Kwok

AU - Mohamad Ali, Irwan

AU - Yeoh, Zi Ning

AU - Mohd Ishak, Lailyvia

AU - Mohd Radzi, Nur Rabiatuladawiah

AU - Hussin, Noor Hamidah

PY - 2012/12

Y1 - 2012/12

N2 - Objective: The capillary electrophoresis (CE) is a new system that utilizes the principle of electrokinetic separation of molecules in eight electrolyte buffer-filled silica capillaries. In this study, we established the normal ranges of haemoglobin A2 (HbA2) and haemoglobin F (HbF) levels for normal individuals using this system and also the HbA2 level in β thalassaemia and haemoglobin E (HbE) individuals. Materials and Methods: 154 samples from normal individuals, 218 samples from β thalassaemia heterozygotes and 91 samples from HbE heterozygotes were subjected to high performance liquid chromatography (HPLC) and CE analysis. Results: The normal ranges for HbA2 and HbF by CE were 2.75% (SD 0.26%) and 0.03% (SD 0.24%) respectively, which were significantly lower than that of HPLC 2.88% (SD 0.25%) and 0.58% (SD 0.61%) (p <0.001). The HbA2 level for HbE heterozygotes was 3.58% (SD 0.44%), which was significantly higher than normal (p <0.001) but lower than that of β-thalassaemia heterozygotes (p<0.001) and the true HbE level was 24.28% (SD 3.38%). Conclusion: The CE system provided a fully automated and high throughput system for haemoglobin analysis. We established the normal ranges for HbA2 and HbF levels by CE. We also determined the ranges for HbA2 in beta thalassaemia and HbE heterozygotes using this system.

AB - Objective: The capillary electrophoresis (CE) is a new system that utilizes the principle of electrokinetic separation of molecules in eight electrolyte buffer-filled silica capillaries. In this study, we established the normal ranges of haemoglobin A2 (HbA2) and haemoglobin F (HbF) levels for normal individuals using this system and also the HbA2 level in β thalassaemia and haemoglobin E (HbE) individuals. Materials and Methods: 154 samples from normal individuals, 218 samples from β thalassaemia heterozygotes and 91 samples from HbE heterozygotes were subjected to high performance liquid chromatography (HPLC) and CE analysis. Results: The normal ranges for HbA2 and HbF by CE were 2.75% (SD 0.26%) and 0.03% (SD 0.24%) respectively, which were significantly lower than that of HPLC 2.88% (SD 0.25%) and 0.58% (SD 0.61%) (p <0.001). The HbA2 level for HbE heterozygotes was 3.58% (SD 0.44%), which was significantly higher than normal (p <0.001) but lower than that of β-thalassaemia heterozygotes (p<0.001) and the true HbE level was 24.28% (SD 3.38%). Conclusion: The CE system provided a fully automated and high throughput system for haemoglobin analysis. We established the normal ranges for HbA2 and HbF levels by CE. We also determined the ranges for HbA2 in beta thalassaemia and HbE heterozygotes using this system.

KW - βthalassaemia

KW - Capillary electrophoresis

KW - Haemoglobin E

KW - HbA

KW - Normal range

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