Haplotype and network analysis of island flying fox (Pteropus hypomelanus) using D-loop region of mitochondrial DNA to confirm subspecies designation

Nur Syafika Mohd-Yusof, Juliana Senawi, Shukor Md Nor, Badrul Munir Md-Zain

Research output: Contribution to journalArticle

Abstract

Taxonomic confusion among island flying foxes (Pteropus hypomelanus) still in debate as in the lack of further genetic studies that describe subspecies validity needs verification. On the basis of morphological variants, 17 subspecies of P. hypomelanus are currently recognized. A previous study showed the distribution of two subspecies of P. hypomelanus in Malaysia: P. hypomelanus robinsoni in Pangkor Island, west coast of Peninsular Malaysia and P. hypomelanus lepidus from Perhentian Island, Kapas Island, and Tioman Island, east coast of Peninsular Malaysia. Herein, using the displacement loop (D-loop) region of mitochondrial DNA sequences, phylogenetic and haplotype network was conducted on their population structure in Peninsular Malaysia upon 42 genetic samples from Dangli Island (Langkawi), Pangkor Island (Perak), Redang Island (Terengganu), and Tinggi Island (Johor). Phylogenetically, two main clades separating northwest and southeast populations emerged. No haplotype was shared between the populations. The results from genetic differentiation (estimate of population subdivision (FST) and nucleotide subdivision (Nst)) yielded a gene flow that was nearly cut-off because of the central mountain that forms as an effective barrier between islands of the east and west coasts of Peninsular Malaysia. Although the above finding genetically confirms their subspecies designation, examination using a nuclear marker is needed in future studies.

Original languageEnglish
JournalMammal Research
DOIs
Publication statusAccepted/In press - 1 Jan 2019

Fingerprint

Pteropus
Pteropodidae
network analysis
mitochondrial DNA
subspecies
haplotypes
Malaysia
coasts
coast
barrier island
founder effect
genetic differentiation
gene flow
population structure
nucleotides
phylogenetics
mountains

Keywords

  • D-loop
  • Haplotype
  • Island flying fox
  • Phylogeny
  • Pteropus hypomelanus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

Cite this

Haplotype and network analysis of island flying fox (Pteropus hypomelanus) using D-loop region of mitochondrial DNA to confirm subspecies designation. / Mohd-Yusof, Nur Syafika; Senawi, Juliana; Nor, Shukor Md; Md-Zain, Badrul Munir.

In: Mammal Research, 01.01.2019.

Research output: Contribution to journalArticle

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