Halophilic glutamate dehydrogenase activities of halobacterium salinarum strain NRC-36014 cultured in different growth conditions

Izyanti Ibrahim, Paul C. Engel

Research output: Contribution to journalArticle

Abstract

The discovery of four glutamate dehydrogense(s) in the genome of a colourless extreme halophile, H. salinarum strain NRC-36014 raised the question regarding the role of these enzymes in this halophile’s metabolism. NRC-36014 was firstly cultured in different conditions, in terms of temperature, salt concentration and nitrogen source, to establish the pattern of expression of these GDH(s). A basic chemically defined medium for NRC-36014 was also generated as no suitable CDM was available. The optimum salt concentration and percentage for NRC-36014 growth is at 4 to 4.5M NaCl and between 20 to 30%, respectively. As for the growth temperature, NRC-36014 favours between 37°C and 45°C. NRC-36014 grown in a range of salt percentages displayed two different NAD+-GDH activities. NADP+-GDH activity was increased in NRC-36014 grown in high NH4Cl concentrations. However, there were two different NAD+-GDH activity observed too, with a possibility that one of them is from a dual-specificity GDH.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalMalaysian Journal of Biochemistry and Molecular Biology
Volume21
Issue number1
Publication statusPublished - 1 Apr 2018

Fingerprint

Halobacterium salinarum
Glutamate Dehydrogenase
Salts
NAD
Halobacteriales
Growth
Temperature
Growth temperature
NADP
Metabolism
Glutamic Acid
Nitrogen
Genes
Genome
Enzymes

Keywords

  • Chemically defined medium
  • Glutamate dehydrogenase
  • Halobacterium salinarum
  • Halophile growth conditions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

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abstract = "The discovery of four glutamate dehydrogense(s) in the genome of a colourless extreme halophile, H. salinarum strain NRC-36014 raised the question regarding the role of these enzymes in this halophile’s metabolism. NRC-36014 was firstly cultured in different conditions, in terms of temperature, salt concentration and nitrogen source, to establish the pattern of expression of these GDH(s). A basic chemically defined medium for NRC-36014 was also generated as no suitable CDM was available. The optimum salt concentration and percentage for NRC-36014 growth is at 4 to 4.5M NaCl and between 20 to 30{\%}, respectively. As for the growth temperature, NRC-36014 favours between 37°C and 45°C. NRC-36014 grown in a range of salt percentages displayed two different NAD+-GDH activities. NADP+-GDH activity was increased in NRC-36014 grown in high NH4Cl concentrations. However, there were two different NAD+-GDH activity observed too, with a possibility that one of them is from a dual-specificity GDH.",
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N2 - The discovery of four glutamate dehydrogense(s) in the genome of a colourless extreme halophile, H. salinarum strain NRC-36014 raised the question regarding the role of these enzymes in this halophile’s metabolism. NRC-36014 was firstly cultured in different conditions, in terms of temperature, salt concentration and nitrogen source, to establish the pattern of expression of these GDH(s). A basic chemically defined medium for NRC-36014 was also generated as no suitable CDM was available. The optimum salt concentration and percentage for NRC-36014 growth is at 4 to 4.5M NaCl and between 20 to 30%, respectively. As for the growth temperature, NRC-36014 favours between 37°C and 45°C. NRC-36014 grown in a range of salt percentages displayed two different NAD+-GDH activities. NADP+-GDH activity was increased in NRC-36014 grown in high NH4Cl concentrations. However, there were two different NAD+-GDH activity observed too, with a possibility that one of them is from a dual-specificity GDH.

AB - The discovery of four glutamate dehydrogense(s) in the genome of a colourless extreme halophile, H. salinarum strain NRC-36014 raised the question regarding the role of these enzymes in this halophile’s metabolism. NRC-36014 was firstly cultured in different conditions, in terms of temperature, salt concentration and nitrogen source, to establish the pattern of expression of these GDH(s). A basic chemically defined medium for NRC-36014 was also generated as no suitable CDM was available. The optimum salt concentration and percentage for NRC-36014 growth is at 4 to 4.5M NaCl and between 20 to 30%, respectively. As for the growth temperature, NRC-36014 favours between 37°C and 45°C. NRC-36014 grown in a range of salt percentages displayed two different NAD+-GDH activities. NADP+-GDH activity was increased in NRC-36014 grown in high NH4Cl concentrations. However, there were two different NAD+-GDH activity observed too, with a possibility that one of them is from a dual-specificity GDH.

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