Halal market surveillance of soft and hard gel capsules in pharmaceutical products using PCR and southern-hybridization on the biochip analysis

Sahilah Abd. Mutalib, Mohd L. Fadly, Norrakiah Abdullah Sani, Aminah Abdullah, Wan Aida Wan Mustapha, Ma`Aruf Abd. Ghani, Mohd. Khan Ayob

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The study was conducted to detect the porcine DNA in pharmaceutical products in local market using polymerase chain reaction (PCR) and southern-hybridization on the biochip. A total of 113 (n=113) of hard (82 samples) and soft gel (31 samples) capsules from pharmaceutical products were purchased and tested for the presence of porcine DNA for Halal authentication. All capsules were gelatin-based purchased from local over the counter (OTC) markets. Of all samples tested, 37.2% (42/113) contained porcine DNA. While, none porcine DNA band was detected for 62.8% (71/113) of capsules tested. All samples which were positive toward porcine DNA were imported pharmaceutical products with none Halal logo. Results in the presence study demonstrated that the PCR techniques and southern-hybridization on the biochip is suitable tool for monitoring the Haram component in highly processed product of soft and hard capsule.

Original languageEnglish
Pages (from-to)371-375
Number of pages5
JournalInternational Food Research Journal
Volume19
Issue number1
Publication statusPublished - 2012

Fingerprint

Southern blotting
Capsules
Swine
Gels
polymerase chain reaction
gels
markets
drugs
Polymerase Chain Reaction
swine
monitoring
DNA
Pharmaceutical Preparations
sampling
Gelatin
gelatin
methodology

Keywords

  • Halal market surveillance
  • PCR and southern-hybridization on the biochip analysis
  • Pharmaceutical products
  • Soft and hard gel capsules

ASJC Scopus subject areas

  • Food Science

Cite this

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title = "Halal market surveillance of soft and hard gel capsules in pharmaceutical products using PCR and southern-hybridization on the biochip analysis",
abstract = "The study was conducted to detect the porcine DNA in pharmaceutical products in local market using polymerase chain reaction (PCR) and southern-hybridization on the biochip. A total of 113 (n=113) of hard (82 samples) and soft gel (31 samples) capsules from pharmaceutical products were purchased and tested for the presence of porcine DNA for Halal authentication. All capsules were gelatin-based purchased from local over the counter (OTC) markets. Of all samples tested, 37.2{\%} (42/113) contained porcine DNA. While, none porcine DNA band was detected for 62.8{\%} (71/113) of capsules tested. All samples which were positive toward porcine DNA were imported pharmaceutical products with none Halal logo. Results in the presence study demonstrated that the PCR techniques and southern-hybridization on the biochip is suitable tool for monitoring the Haram component in highly processed product of soft and hard capsule.",
keywords = "Halal market surveillance, PCR and southern-hybridization on the biochip analysis, Pharmaceutical products, Soft and hard gel capsules",
author = "{Abd. Mutalib}, Sahilah and Fadly, {Mohd L.} and {Abdullah Sani}, Norrakiah and Aminah Abdullah and {Wan Mustapha}, {Wan Aida} and {Abd. Ghani}, Ma`Aruf and Ayob, {Mohd. Khan}",
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AU - Abd. Mutalib, Sahilah

AU - Fadly, Mohd L.

AU - Abdullah Sani, Norrakiah

AU - Abdullah, Aminah

AU - Wan Mustapha, Wan Aida

AU - Abd. Ghani, Ma`Aruf

AU - Ayob, Mohd. Khan

PY - 2012

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AB - The study was conducted to detect the porcine DNA in pharmaceutical products in local market using polymerase chain reaction (PCR) and southern-hybridization on the biochip. A total of 113 (n=113) of hard (82 samples) and soft gel (31 samples) capsules from pharmaceutical products were purchased and tested for the presence of porcine DNA for Halal authentication. All capsules were gelatin-based purchased from local over the counter (OTC) markets. Of all samples tested, 37.2% (42/113) contained porcine DNA. While, none porcine DNA band was detected for 62.8% (71/113) of capsules tested. All samples which were positive toward porcine DNA were imported pharmaceutical products with none Halal logo. Results in the presence study demonstrated that the PCR techniques and southern-hybridization on the biochip is suitable tool for monitoring the Haram component in highly processed product of soft and hard capsule.

KW - Halal market surveillance

KW - PCR and southern-hybridization on the biochip analysis

KW - Pharmaceutical products

KW - Soft and hard gel capsules

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