Guided bone regeneration using autologous plasma, bone marrow cells and β-TCP/HA granules for experimental alveolar ridge reconstruction in Macaca fascicularis

Sharen Aini Shamsuddin, Roszalina Ramli, Masfueh Razali, Badiah Baharin, Shamsul Sulaiman, Min Hwei Ng, Cheong Kiat Low, Mohd Nazimi Abd Jabar, Rifqah Nordin, Norziha Yahaya, Shariffah Shuriana Abdul Shukur, Chong Huat Siar, Ruszymah Idrus

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Abstract

Alveolar bone loss is a common problem involving all age groups. The purpose of this study was to regenerate the alveolar bone via tissue engineering technique using autologous bone marrow-derived stem cells (BMSCs). Autologous BMSCs were mixed with fibrin and seeded onto beta-tricalcium phosphate and hydroxyapatite (β-TCP/HA) granules to induce bone formation and alveolar ridge restoration in an animal model, Macaca fascicularis. BMSCs were aspirated from the femur and cultured in alpha-minimal essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS) and 5% autologous serum. Approximately 3×107 cells were suspended in fibrin before seeding onto β-TCP/HA granules and incubated for 24 hours for cell attachment. A defect area, measuring 1 cm×1 cm×0.7 cm was created in both test and control animals. Cell-seeded β-TCP/HA granule were implanted into the defect area in test animals (n = 6) and the defect was left to heal without any intervention in the controls (n = 4), After 12 weeks, the animals were sacrificed. Scanning electron microscopic evaluation of the alveolar ridge demonstrated the presence of collagen matrix and osteoblast-like cells in the defect area indicating new bone formation. Micro computerized tomography (micro-CT scan) analysis confirmed the presence of dense bone tissues around the β-TCP/HA granules. The alveolar bone measurement showed a significant increment of the bone height from 2.75±0.52 mm to 8.00±0.36 mm with p <0.05. Histological section stained with hematoxylin and eosin showed good integration between new and native bone. BMSCs seeded β-TCP/HA is an option to increase alveolar ridge height and width shown in a Macaca fascicularis model.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalJournal of Biomaterials and Tissue Engineering
Volume7
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017

Fingerprint

Alveolar Process
Bone Regeneration
Macaca fascicularis
Bone Marrow Cells
Bone
Cells
Plasmas
Bone and Bones
Stem Cells
Bone Marrow
Fibrin
Osteogenesis
Stem cells
Alveolar Bone Loss
Animals
Hematoxylin
Tissue Engineering
Eosine Yellowish-(YS)
Defects
Serum

Keywords

  • Alveolar Ridge
  • Macaca fascicularis
  • Stem Cells
  • Tissue Engineering
  • β-TCP/HA Granules

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering

Cite this

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title = "Guided bone regeneration using autologous plasma, bone marrow cells and β-TCP/HA granules for experimental alveolar ridge reconstruction in Macaca fascicularis",
abstract = "Alveolar bone loss is a common problem involving all age groups. The purpose of this study was to regenerate the alveolar bone via tissue engineering technique using autologous bone marrow-derived stem cells (BMSCs). Autologous BMSCs were mixed with fibrin and seeded onto beta-tricalcium phosphate and hydroxyapatite (β-TCP/HA) granules to induce bone formation and alveolar ridge restoration in an animal model, Macaca fascicularis. BMSCs were aspirated from the femur and cultured in alpha-minimal essential medium (α-MEM) supplemented with 10{\%} fetal bovine serum (FBS) and 5{\%} autologous serum. Approximately 3×107 cells were suspended in fibrin before seeding onto β-TCP/HA granules and incubated for 24 hours for cell attachment. A defect area, measuring 1 cm×1 cm×0.7 cm was created in both test and control animals. Cell-seeded β-TCP/HA granule were implanted into the defect area in test animals (n = 6) and the defect was left to heal without any intervention in the controls (n = 4), After 12 weeks, the animals were sacrificed. Scanning electron microscopic evaluation of the alveolar ridge demonstrated the presence of collagen matrix and osteoblast-like cells in the defect area indicating new bone formation. Micro computerized tomography (micro-CT scan) analysis confirmed the presence of dense bone tissues around the β-TCP/HA granules. The alveolar bone measurement showed a significant increment of the bone height from 2.75±0.52 mm to 8.00±0.36 mm with p <0.05. Histological section stained with hematoxylin and eosin showed good integration between new and native bone. BMSCs seeded β-TCP/HA is an option to increase alveolar ridge height and width shown in a Macaca fascicularis model.",
keywords = "Alveolar Ridge, Macaca fascicularis, Stem Cells, Tissue Engineering, β-TCP/HA Granules",
author = "Shamsuddin, {Sharen Aini} and Roszalina Ramli and Masfueh Razali and Badiah Baharin and Shamsul Sulaiman and Ng, {Min Hwei} and Low, {Cheong Kiat} and {Abd Jabar}, {Mohd Nazimi} and Rifqah Nordin and Norziha Yahaya and Shukur, {Shariffah Shuriana Abdul} and Siar, {Chong Huat} and Ruszymah Idrus",
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T1 - Guided bone regeneration using autologous plasma, bone marrow cells and β-TCP/HA granules for experimental alveolar ridge reconstruction in Macaca fascicularis

AU - Shamsuddin, Sharen Aini

AU - Ramli, Roszalina

AU - Razali, Masfueh

AU - Baharin, Badiah

AU - Sulaiman, Shamsul

AU - Ng, Min Hwei

AU - Low, Cheong Kiat

AU - Abd Jabar, Mohd Nazimi

AU - Nordin, Rifqah

AU - Yahaya, Norziha

AU - Shukur, Shariffah Shuriana Abdul

AU - Siar, Chong Huat

AU - Idrus, Ruszymah

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Alveolar bone loss is a common problem involving all age groups. The purpose of this study was to regenerate the alveolar bone via tissue engineering technique using autologous bone marrow-derived stem cells (BMSCs). Autologous BMSCs were mixed with fibrin and seeded onto beta-tricalcium phosphate and hydroxyapatite (β-TCP/HA) granules to induce bone formation and alveolar ridge restoration in an animal model, Macaca fascicularis. BMSCs were aspirated from the femur and cultured in alpha-minimal essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS) and 5% autologous serum. Approximately 3×107 cells were suspended in fibrin before seeding onto β-TCP/HA granules and incubated for 24 hours for cell attachment. A defect area, measuring 1 cm×1 cm×0.7 cm was created in both test and control animals. Cell-seeded β-TCP/HA granule were implanted into the defect area in test animals (n = 6) and the defect was left to heal without any intervention in the controls (n = 4), After 12 weeks, the animals were sacrificed. Scanning electron microscopic evaluation of the alveolar ridge demonstrated the presence of collagen matrix and osteoblast-like cells in the defect area indicating new bone formation. Micro computerized tomography (micro-CT scan) analysis confirmed the presence of dense bone tissues around the β-TCP/HA granules. The alveolar bone measurement showed a significant increment of the bone height from 2.75±0.52 mm to 8.00±0.36 mm with p <0.05. Histological section stained with hematoxylin and eosin showed good integration between new and native bone. BMSCs seeded β-TCP/HA is an option to increase alveolar ridge height and width shown in a Macaca fascicularis model.

AB - Alveolar bone loss is a common problem involving all age groups. The purpose of this study was to regenerate the alveolar bone via tissue engineering technique using autologous bone marrow-derived stem cells (BMSCs). Autologous BMSCs were mixed with fibrin and seeded onto beta-tricalcium phosphate and hydroxyapatite (β-TCP/HA) granules to induce bone formation and alveolar ridge restoration in an animal model, Macaca fascicularis. BMSCs were aspirated from the femur and cultured in alpha-minimal essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS) and 5% autologous serum. Approximately 3×107 cells were suspended in fibrin before seeding onto β-TCP/HA granules and incubated for 24 hours for cell attachment. A defect area, measuring 1 cm×1 cm×0.7 cm was created in both test and control animals. Cell-seeded β-TCP/HA granule were implanted into the defect area in test animals (n = 6) and the defect was left to heal without any intervention in the controls (n = 4), After 12 weeks, the animals were sacrificed. Scanning electron microscopic evaluation of the alveolar ridge demonstrated the presence of collagen matrix and osteoblast-like cells in the defect area indicating new bone formation. Micro computerized tomography (micro-CT scan) analysis confirmed the presence of dense bone tissues around the β-TCP/HA granules. The alveolar bone measurement showed a significant increment of the bone height from 2.75±0.52 mm to 8.00±0.36 mm with p <0.05. Histological section stained with hematoxylin and eosin showed good integration between new and native bone. BMSCs seeded β-TCP/HA is an option to increase alveolar ridge height and width shown in a Macaca fascicularis model.

KW - Alveolar Ridge

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KW - Stem Cells

KW - Tissue Engineering

KW - β-TCP/HA Granules

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