Growth medium optimization for biomass production of a probiotic bacterium, lactobacillus rhamnosus ATCC 7469

C. P. Chang, S. L. Liew

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    This study was undertaken to determine the glucose, yeast extract, tryptone and Tween 80 concentrations in a cultivation medium for optimal biomass production of a probiotic bacterium, Lactobacillus rhamnosusATCC 7469. Using response surface methodology, a central composite rotatable design was applied to allocate treatment combinations. A polynomial regression model containing linear, quadratic, cubic and quartic terms was used for analysis of the experimental data. Effects involving all four factors evaluated were found to be significant and the strongest effect was given by tryptone concentration. Estimated optimum conditions of the factors for the growth of L.rhamnosus were as follows: glucose=4.4% (w/v), yeast extract=6.0% (w/v), tryptone=6.0% (w/v) and Tween 80=1.1mL/L. The optimum-point medium gave higher counts of viable cells, specific growth rate and biomass productivity than de Man, Rogosa and Sharpe and the center-point media. Practical Applications: With increasing demand for food products containing probiotic microorganisms from health-conscious consumers, there arises a need to establish effective fermentation techniques for probiotic biomass production. Medium formulation, fermentation parameters and fermentor design play important roles to ensure optimal results. This study highlights a statistical approach to optimizing medium formulation for a probiotic lactic acid bacteria strain, which is known for its high nutritional demands. The optimal medium formulation obtained from this study can be used for improving Lactobacillus rhamnosus fermentation as it is capable of producing more viable cells than de Man, Rogosa and Sharpe medium, which is generally recognized and used as a suitable medium for lactic acid bacteria cultivation.

    Original languageEnglish
    Pages (from-to)536-543
    Number of pages8
    JournalJournal of Food Biochemistry
    Volume37
    Issue number5
    DOIs
    Publication statusPublished - Oct 2013

    Fingerprint

    Lactobacillus rhamnosus
    Probiotics
    Biomass
    probiotics
    biomass production
    culture media
    Fermentation
    Bacteria
    Polysorbates
    bacteria
    fermentation
    yeast extract
    Growth
    lactic acid bacteria
    Lactic Acid
    Yeasts
    Glucose
    glucose
    fermenters
    Lactobacillus

    ASJC Scopus subject areas

    • Food Science
    • Cell Biology
    • Pharmacology
    • Biophysics

    Cite this

    Growth medium optimization for biomass production of a probiotic bacterium, lactobacillus rhamnosus ATCC 7469. / Chang, C. P.; Liew, S. L.

    In: Journal of Food Biochemistry, Vol. 37, No. 5, 10.2013, p. 536-543.

    Research output: Contribution to journalArticle

    @article{d106e5257f844d47b48eb658d5490cdc,
    title = "Growth medium optimization for biomass production of a probiotic bacterium, lactobacillus rhamnosus ATCC 7469",
    abstract = "This study was undertaken to determine the glucose, yeast extract, tryptone and Tween 80 concentrations in a cultivation medium for optimal biomass production of a probiotic bacterium, Lactobacillus rhamnosusATCC 7469. Using response surface methodology, a central composite rotatable design was applied to allocate treatment combinations. A polynomial regression model containing linear, quadratic, cubic and quartic terms was used for analysis of the experimental data. Effects involving all four factors evaluated were found to be significant and the strongest effect was given by tryptone concentration. Estimated optimum conditions of the factors for the growth of L.rhamnosus were as follows: glucose=4.4{\%} (w/v), yeast extract=6.0{\%} (w/v), tryptone=6.0{\%} (w/v) and Tween 80=1.1mL/L. The optimum-point medium gave higher counts of viable cells, specific growth rate and biomass productivity than de Man, Rogosa and Sharpe and the center-point media. Practical Applications: With increasing demand for food products containing probiotic microorganisms from health-conscious consumers, there arises a need to establish effective fermentation techniques for probiotic biomass production. Medium formulation, fermentation parameters and fermentor design play important roles to ensure optimal results. This study highlights a statistical approach to optimizing medium formulation for a probiotic lactic acid bacteria strain, which is known for its high nutritional demands. The optimal medium formulation obtained from this study can be used for improving Lactobacillus rhamnosus fermentation as it is capable of producing more viable cells than de Man, Rogosa and Sharpe medium, which is generally recognized and used as a suitable medium for lactic acid bacteria cultivation.",
    author = "Chang, {C. P.} and Liew, {S. L.}",
    year = "2013",
    month = "10",
    doi = "10.1111/jfbc.12004",
    language = "English",
    volume = "37",
    pages = "536--543",
    journal = "Journal of Food Biochemistry",
    issn = "0145-8884",
    publisher = "Wiley-Blackwell",
    number = "5",

    }

    TY - JOUR

    T1 - Growth medium optimization for biomass production of a probiotic bacterium, lactobacillus rhamnosus ATCC 7469

    AU - Chang, C. P.

    AU - Liew, S. L.

    PY - 2013/10

    Y1 - 2013/10

    N2 - This study was undertaken to determine the glucose, yeast extract, tryptone and Tween 80 concentrations in a cultivation medium for optimal biomass production of a probiotic bacterium, Lactobacillus rhamnosusATCC 7469. Using response surface methodology, a central composite rotatable design was applied to allocate treatment combinations. A polynomial regression model containing linear, quadratic, cubic and quartic terms was used for analysis of the experimental data. Effects involving all four factors evaluated were found to be significant and the strongest effect was given by tryptone concentration. Estimated optimum conditions of the factors for the growth of L.rhamnosus were as follows: glucose=4.4% (w/v), yeast extract=6.0% (w/v), tryptone=6.0% (w/v) and Tween 80=1.1mL/L. The optimum-point medium gave higher counts of viable cells, specific growth rate and biomass productivity than de Man, Rogosa and Sharpe and the center-point media. Practical Applications: With increasing demand for food products containing probiotic microorganisms from health-conscious consumers, there arises a need to establish effective fermentation techniques for probiotic biomass production. Medium formulation, fermentation parameters and fermentor design play important roles to ensure optimal results. This study highlights a statistical approach to optimizing medium formulation for a probiotic lactic acid bacteria strain, which is known for its high nutritional demands. The optimal medium formulation obtained from this study can be used for improving Lactobacillus rhamnosus fermentation as it is capable of producing more viable cells than de Man, Rogosa and Sharpe medium, which is generally recognized and used as a suitable medium for lactic acid bacteria cultivation.

    AB - This study was undertaken to determine the glucose, yeast extract, tryptone and Tween 80 concentrations in a cultivation medium for optimal biomass production of a probiotic bacterium, Lactobacillus rhamnosusATCC 7469. Using response surface methodology, a central composite rotatable design was applied to allocate treatment combinations. A polynomial regression model containing linear, quadratic, cubic and quartic terms was used for analysis of the experimental data. Effects involving all four factors evaluated were found to be significant and the strongest effect was given by tryptone concentration. Estimated optimum conditions of the factors for the growth of L.rhamnosus were as follows: glucose=4.4% (w/v), yeast extract=6.0% (w/v), tryptone=6.0% (w/v) and Tween 80=1.1mL/L. The optimum-point medium gave higher counts of viable cells, specific growth rate and biomass productivity than de Man, Rogosa and Sharpe and the center-point media. Practical Applications: With increasing demand for food products containing probiotic microorganisms from health-conscious consumers, there arises a need to establish effective fermentation techniques for probiotic biomass production. Medium formulation, fermentation parameters and fermentor design play important roles to ensure optimal results. This study highlights a statistical approach to optimizing medium formulation for a probiotic lactic acid bacteria strain, which is known for its high nutritional demands. The optimal medium formulation obtained from this study can be used for improving Lactobacillus rhamnosus fermentation as it is capable of producing more viable cells than de Man, Rogosa and Sharpe medium, which is generally recognized and used as a suitable medium for lactic acid bacteria cultivation.

    UR - http://www.scopus.com/inward/record.url?scp=84885481423&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84885481423&partnerID=8YFLogxK

    U2 - 10.1111/jfbc.12004

    DO - 10.1111/jfbc.12004

    M3 - Article

    VL - 37

    SP - 536

    EP - 543

    JO - Journal of Food Biochemistry

    JF - Journal of Food Biochemistry

    SN - 0145-8884

    IS - 5

    ER -