Growth improvement and metabolic profiling of native and commercial Chlorella sorokiniana strains acclimatized in recycled agricultural wastewater

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4 Citations (Scopus)

Abstract

This study investigated acclimation ability of native Chlorella sorokiniana (CS-N) and commercial Chlorella sorokiniana (CS-C) in palm oil mill effluent (POME), their metabolic profile and feasibility of effluent recycling for dilution purpose. Maximum specific growth rate, µmax and lag time, λ of the microalgae were evaluated. Result shows both strains produced comparable growth in POME, with µmax of 0.31 day−1 and 0.30 day−1 respectively, albeit longer λ by the CS-C. However, three cycles of acclimation was able to reduce λ from eight days to two days for CS-C. Metabolic profiling using principal component analysis (PCA) shows clear cluster of acclimatized strains to suggest better stress tolerance of CS-N. Finally, a remarkable µmax of 0.57 day−1 without lag phase was achieved using acclimatized CS-N in 40% POME concentration. Acclimation has successfully shortened the λ and dilution with final effluent was proved to be feasible for further improvement of the microalgae growth.

Original languageEnglish
Pages (from-to)930-939
Number of pages10
JournalBioresource Technology
Volume247
DOIs
Publication statusPublished - 2018

Fingerprint

Effluents
Wastewater
Palm oil
effluent
wastewater
acclimation
mill
Dilution
oil
dilution
Principal component analysis
Recycling
principal component analysis
recycling
tolerance
palm oil

Keywords

  • Chlorella sorokiniana
  • Metabolic profiling
  • Microalgae acclimation
  • Palm oil mill effluent (POME) recycling
  • Principal component analysis (PCA)

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

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title = "Growth improvement and metabolic profiling of native and commercial Chlorella sorokiniana strains acclimatized in recycled agricultural wastewater",
abstract = "This study investigated acclimation ability of native Chlorella sorokiniana (CS-N) and commercial Chlorella sorokiniana (CS-C) in palm oil mill effluent (POME), their metabolic profile and feasibility of effluent recycling for dilution purpose. Maximum specific growth rate, µmax and lag time, λ of the microalgae were evaluated. Result shows both strains produced comparable growth in POME, with µmax of 0.31 day−1 and 0.30 day−1 respectively, albeit longer λ by the CS-C. However, three cycles of acclimation was able to reduce λ from eight days to two days for CS-C. Metabolic profiling using principal component analysis (PCA) shows clear cluster of acclimatized strains to suggest better stress tolerance of CS-N. Finally, a remarkable µmax of 0.57 day−1 without lag phase was achieved using acclimatized CS-N in 40{\%} POME concentration. Acclimation has successfully shortened the λ and dilution with final effluent was proved to be feasible for further improvement of the microalgae growth.",
keywords = "Chlorella sorokiniana, Metabolic profiling, Microalgae acclimation, Palm oil mill effluent (POME) recycling, Principal component analysis (PCA)",
author = "Khalid, {Azianabiha A.Halip} and Zahira Yaakob and {Sheikh Abdullah}, {Siti Rozaimah} and Takriff, {Mohd Sobri}",
year = "2018",
doi = "10.1016/j.biortech.2017.09.195",
language = "English",
volume = "247",
pages = "930--939",
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T1 - Growth improvement and metabolic profiling of native and commercial Chlorella sorokiniana strains acclimatized in recycled agricultural wastewater

AU - Khalid, Azianabiha A.Halip

AU - Yaakob, Zahira

AU - Sheikh Abdullah, Siti Rozaimah

AU - Takriff, Mohd Sobri

PY - 2018

Y1 - 2018

N2 - This study investigated acclimation ability of native Chlorella sorokiniana (CS-N) and commercial Chlorella sorokiniana (CS-C) in palm oil mill effluent (POME), their metabolic profile and feasibility of effluent recycling for dilution purpose. Maximum specific growth rate, µmax and lag time, λ of the microalgae were evaluated. Result shows both strains produced comparable growth in POME, with µmax of 0.31 day−1 and 0.30 day−1 respectively, albeit longer λ by the CS-C. However, three cycles of acclimation was able to reduce λ from eight days to two days for CS-C. Metabolic profiling using principal component analysis (PCA) shows clear cluster of acclimatized strains to suggest better stress tolerance of CS-N. Finally, a remarkable µmax of 0.57 day−1 without lag phase was achieved using acclimatized CS-N in 40% POME concentration. Acclimation has successfully shortened the λ and dilution with final effluent was proved to be feasible for further improvement of the microalgae growth.

AB - This study investigated acclimation ability of native Chlorella sorokiniana (CS-N) and commercial Chlorella sorokiniana (CS-C) in palm oil mill effluent (POME), their metabolic profile and feasibility of effluent recycling for dilution purpose. Maximum specific growth rate, µmax and lag time, λ of the microalgae were evaluated. Result shows both strains produced comparable growth in POME, with µmax of 0.31 day−1 and 0.30 day−1 respectively, albeit longer λ by the CS-C. However, three cycles of acclimation was able to reduce λ from eight days to two days for CS-C. Metabolic profiling using principal component analysis (PCA) shows clear cluster of acclimatized strains to suggest better stress tolerance of CS-N. Finally, a remarkable µmax of 0.57 day−1 without lag phase was achieved using acclimatized CS-N in 40% POME concentration. Acclimation has successfully shortened the λ and dilution with final effluent was proved to be feasible for further improvement of the microalgae growth.

KW - Chlorella sorokiniana

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KW - Palm oil mill effluent (POME) recycling

KW - Principal component analysis (PCA)

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