Growth and survival of Cronobacter species as measured by media performance

M. Ghassem, Abd. Salam Babji, S. J. Forsythe, Norrakiah Abdullah Sani

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cronobacter sakazakii is an emerging food borne pathogen which has been associated with outbreaks of a rare form of infant meningitis. Although the origin of the microorganism has not been established, several infection cases have been associated with the consumption of contaminated powdered infant formula (PIF). In the present study, growth characteristics of three C. sakazakii strains isolated from PIF samples and C. muytjensii strain ATCC 51329, which was formerly the ATCC Preceptrol™ strain for the quality control of 'Enterobacter sakazakii' prior to the taxonomic revision, were investigated in Tryptone Soya broth (TSB) and reconstituted PIF at 4, 10, 25, 37, 45 and 50°C. The viability of heat treated cells of Cronobacter strains was evaluated by plating on Violet Red Bile Glucose agar (VRBGA) and the Druggan-Forsythe-Iversen (DFI) chromogenic agar followed by incubation at 37°C. These strains were also subjected to higher temperatures between 52 to 60°C to measure their thermal tolerance. The mean generation time of all Cronobacter strains were slightly lower in PIF than in TSB. C. muytjensii ATCC 51329 showed lower generation time in all culture media and all temperatures compared to the Cronobacter food isolates, but the results were not significantly different (P>0.05). The results also indicated that combination of PIF: DFI culture media had higher recovery at all temperatures compared to other combinations. Survival study also indicated that C. muytjensii ATCC 51329 had higher D-value compared to food isolates at all incubation temperatures.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalInternational Food Research Journal
Volume18
Issue number1
Publication statusPublished - 2011

Fingerprint

Cronobacter
Infant Formula
Cronobacter sakazakii
infant formulas
Growth
Temperature
Food
Agar
Culture Media
Viola
temperature
culture media
agar
Meningitis
Bile
Quality Control
meningitis
Disease Outbreaks
food pathogens
bile

Keywords

  • Chromogenic agar
  • Cronobacter sakazakii
  • Generation time
  • Powdered infant formula
  • Thermotolerance

ASJC Scopus subject areas

  • Food Science

Cite this

Growth and survival of Cronobacter species as measured by media performance. / Ghassem, M.; Babji, Abd. Salam; Forsythe, S. J.; Abdullah Sani, Norrakiah.

In: International Food Research Journal, Vol. 18, No. 1, 2011, p. 367-372.

Research output: Contribution to journalArticle

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