Growth and differentiation potentials in confluent state of culture of human skeletal muscle myoblasts

Shiplu Roy Chowdhury, Yuichi Muneyuki, Yasunori Takezawa, Masahiro Kino-oka, Atsuhiro Saito, Yoshiki Sawa, Masahito Taya

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The transitional behaviors of myoblasts toward differentiation were investigated in the cultures at the low and high seeding densities (respectively, X0 = 1.0 × 103 and 2.0 × 105 cells/cm2). In the culture at the low seeding density, an increase in confluence degree accompanied a decrease in growth potential (Rp), being Rp = 0.85 and 0.11 at t = 48 and 672 h, respectively. Myoblasts seeded at the high density resulted in the immediate cessation of growth with keeping the low range of Rp = 0.02-0.09 throughout the culture. The reduction of Rp led to the generation of three subpopulations of cells in proliferative, quiescent and differentiated states. Close cell contacts in the confluent state of high seeding culture induced cell quiescence to a higher extent with suppressing differentiation.

Original languageEnglish
Pages (from-to)310-313
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume109
Issue number3
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

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Skeletal Myoblasts
Muscle
Skeletal Muscle
Myoblasts
Growth
Cells
Cell Culture Techniques

Keywords

  • Confluent state
  • Differentiation
  • Myoblasts
  • Quiescence
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Growth and differentiation potentials in confluent state of culture of human skeletal muscle myoblasts. / Chowdhury, Shiplu Roy; Muneyuki, Yuichi; Takezawa, Yasunori; Kino-oka, Masahiro; Saito, Atsuhiro; Sawa, Yoshiki; Taya, Masahito.

In: Journal of Bioscience and Bioengineering, Vol. 109, No. 3, 03.2010, p. 310-313.

Research output: Contribution to journalArticle

Chowdhury, Shiplu Roy ; Muneyuki, Yuichi ; Takezawa, Yasunori ; Kino-oka, Masahiro ; Saito, Atsuhiro ; Sawa, Yoshiki ; Taya, Masahito. / Growth and differentiation potentials in confluent state of culture of human skeletal muscle myoblasts. In: Journal of Bioscience and Bioengineering. 2010 ; Vol. 109, No. 3. pp. 310-313.
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