Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract

Ghassan Mohammad Sulaiman, Wasnaa Hatif Mohammed, Thorria Radam Marzoog, Ahmed Abdul Amir Al-Amiery, Abdul Amir H. Kadhum, Abu Bakar Mohamad

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Objective: To synthesize silver nanopaticles from leaves extract of Eucalyptus chapmaniana (E. chapmaniana) and test the antimicrobial of the nanoparticles against different pathogenic bacteria, yeast and its toxicity against human acute promyelocytic leukemia (HL-60) cell line. Methods: Ten milliliter of leaves extract was mixed with 90 mL of 0.01 mmol/mL or 0.02 mmol/mL aqueous AgNO3 and exposed to sun light for 1 h. A change from yellowish to reddish brown color was observed. Characterization using UV-vis spectrophotometery and X-ray diffraction analysis were performed. Antimicrobial activity against six microorganisms was tested using well diffusion method and cytoxicity test using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole was obtained on the human leukemia cell line (HL-60). Results: UV-vis spectral analysis showed silver surface plasmon resonance band at 413 nm. X-ray diffraction showed that the particles were crystalline in nature with face centered cubic structure of the bulk silver with broad beaks at 38.50° and 44.76°. The synthesized silver nanoparticles efficiently inhibited various pathogenic organisms and reduced viability of the HL-60 cells in a dose-dependent manner. Conclusions: It has been demonstrated that the extract of E. chapmaniana leaves are capable of producing silver nanoparticles extracellularly and the Ag nanoparticles are quite stable in solution. Further studies are needed to fully characterize the toxicity and the mechanisms involved with the antimicrobial and anticancer activity of these particles.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalAsian Pacific Journal of Tropical Biomedicine
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Eucalyptus
Silver
Nanoparticles
HL-60 Cells
X-Ray Diffraction
Toxicity
Cells
Beak
Cell Line
Acute Promyelocytic Leukemia
Surface Plasmon Resonance
Surface plasmon resonance
Solar System
Microorganisms
Sun
Yeast
Spectrum analysis
X ray diffraction analysis
Bacteria
Leukemia

Keywords

  • Antimicrobial activity
  • Cytotoxic effect
  • Eucalyptus chapmaniana
  • Silver nanoparticles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. / Sulaiman, Ghassan Mohammad; Mohammed, Wasnaa Hatif; Marzoog, Thorria Radam; Al-Amiery, Ahmed Abdul Amir; Kadhum, Abdul Amir H.; Mohamad, Abu Bakar.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 3, No. 1, 01.2013, p. 58-63.

Research output: Contribution to journalArticle

Sulaiman, Ghassan Mohammad ; Mohammed, Wasnaa Hatif ; Marzoog, Thorria Radam ; Al-Amiery, Ahmed Abdul Amir ; Kadhum, Abdul Amir H. ; Mohamad, Abu Bakar. / Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. In: Asian Pacific Journal of Tropical Biomedicine. 2013 ; Vol. 3, No. 1. pp. 58-63.
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