Grain yield QTLs with consistent-effect under reproductive-stage drought stress in rice

Alvin D. Palanog, B. P Mallikarjuna Swamy, Noraziyah Abd Aziz Shamsudin, Shalabh Dixit, Jose E. Hernandez, Teresita H. Boromeo, Pompe C Sta Cruz, Arvind Kumar

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study, consistent-effect QTLs associated with grain yield under drought stress were identified using two mapping populations derived from crossing Kali Aus, a drought-tolerant rice landrace from India with mega varieties IR64 and MTU1010 as recipient parents. Two populations were phenotyped for yield and yield-component traits under reproductive-stage drought-stress and non-stress conditions in the 2011 and 2012 dry seasons (DS) at IRRI. Bulk-segregant analysis, followed by whole genotyping with selected markers, was used to identify significant markers putatively linked with high grain yield under drought. Six polymorphic markers RM246, RM450, RM250, RM232, RM518, and RM19 were found significant in bulk segregant analysis (BSA) of the Kali Aus/IR64 mapping population. Eight polymorphic markers RM495, RM572, RM246, RM211, RM250, RM231, RM3, and RM340 were found significant in the Kali Aus/MTU1010 mapping population. Analysis revealed major-effect grain yield QTLs qDTY2.3 and qDTY2.2 under drought stress consistently over two seasons in the Kali Aus/IR64 and Kali Aus/MTU1010 populations, respectively. qDTY2.3 explained 9% of phenotypic variance, with an additive effect of 451kgha-1 whereas qDTY2.2 explained 6% of phenotypic variance, with an additive effect of 121kgha-1. The QTLs showed consistent effect over two years of combined stress conditions. The two consistent-effect QTLs qDTY2.2 and qDTY2.3 can be used to improve the grain yield of mega varieties IR64 and MTU1010, respectively, across varying severity of drought stress through marker-aided backcrossing and thus provide farmers with improved varieties that effectively combines high yield potential with good yield under drought.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalField Crops Research
Volume161
DOIs
Publication statusPublished - 2014

Fingerprint

drought stress
quantitative trait loci
water stress
rice
grain yield
drought
additive effect
phenotypic variation
backcrossing
yield components
landraces
genotyping
dry season
effect
farmers
India
cultivars
marker

Keywords

  • Bulk segregant analysis
  • Drought
  • Marker-assisted breeding
  • Quantitative trait loci

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Cite this

Palanog, A. D., Swamy, B. P. M., Abd Aziz Shamsudin, N., Dixit, S., Hernandez, J. E., Boromeo, T. H., ... Kumar, A. (2014). Grain yield QTLs with consistent-effect under reproductive-stage drought stress in rice. Field Crops Research, 161, 46-54. https://doi.org/10.1016/j.fcr.2014.01.004

Grain yield QTLs with consistent-effect under reproductive-stage drought stress in rice. / Palanog, Alvin D.; Swamy, B. P Mallikarjuna; Abd Aziz Shamsudin, Noraziyah; Dixit, Shalabh; Hernandez, Jose E.; Boromeo, Teresita H.; Cruz, Pompe C Sta; Kumar, Arvind.

In: Field Crops Research, Vol. 161, 2014, p. 46-54.

Research output: Contribution to journalArticle

Palanog, AD, Swamy, BPM, Abd Aziz Shamsudin, N, Dixit, S, Hernandez, JE, Boromeo, TH, Cruz, PCS & Kumar, A 2014, 'Grain yield QTLs with consistent-effect under reproductive-stage drought stress in rice', Field Crops Research, vol. 161, pp. 46-54. https://doi.org/10.1016/j.fcr.2014.01.004
Palanog, Alvin D. ; Swamy, B. P Mallikarjuna ; Abd Aziz Shamsudin, Noraziyah ; Dixit, Shalabh ; Hernandez, Jose E. ; Boromeo, Teresita H. ; Cruz, Pompe C Sta ; Kumar, Arvind. / Grain yield QTLs with consistent-effect under reproductive-stage drought stress in rice. In: Field Crops Research. 2014 ; Vol. 161. pp. 46-54.
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AU - Swamy, B. P Mallikarjuna

AU - Abd Aziz Shamsudin, Noraziyah

AU - Dixit, Shalabh

AU - Hernandez, Jose E.

AU - Boromeo, Teresita H.

AU - Cruz, Pompe C Sta

AU - Kumar, Arvind

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AB - In this study, consistent-effect QTLs associated with grain yield under drought stress were identified using two mapping populations derived from crossing Kali Aus, a drought-tolerant rice landrace from India with mega varieties IR64 and MTU1010 as recipient parents. Two populations were phenotyped for yield and yield-component traits under reproductive-stage drought-stress and non-stress conditions in the 2011 and 2012 dry seasons (DS) at IRRI. Bulk-segregant analysis, followed by whole genotyping with selected markers, was used to identify significant markers putatively linked with high grain yield under drought. Six polymorphic markers RM246, RM450, RM250, RM232, RM518, and RM19 were found significant in bulk segregant analysis (BSA) of the Kali Aus/IR64 mapping population. Eight polymorphic markers RM495, RM572, RM246, RM211, RM250, RM231, RM3, and RM340 were found significant in the Kali Aus/MTU1010 mapping population. Analysis revealed major-effect grain yield QTLs qDTY2.3 and qDTY2.2 under drought stress consistently over two seasons in the Kali Aus/IR64 and Kali Aus/MTU1010 populations, respectively. qDTY2.3 explained 9% of phenotypic variance, with an additive effect of 451kgha-1 whereas qDTY2.2 explained 6% of phenotypic variance, with an additive effect of 121kgha-1. The QTLs showed consistent effect over two years of combined stress conditions. The two consistent-effect QTLs qDTY2.2 and qDTY2.3 can be used to improve the grain yield of mega varieties IR64 and MTU1010, respectively, across varying severity of drought stress through marker-aided backcrossing and thus provide farmers with improved varieties that effectively combines high yield potential with good yield under drought.

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