Glyphosate resistance in Eleusine indica (L.) Gaertn. from different origins and polymerase chain reaction amplification of specific alleles

C. H. Ng, R Wickneswari V Ratnam, S. Salmijah, Y. T. Teng, Ismail Sahid

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38 Citations (Scopus)

Abstract

Glyphosate-resistant populations of Eleusine indica (L.) Gaertn. were found in 4 areas in Malaysia that had received repeated applications of glyphosate for a period ranging from 5 to 15 years. The resistance ratios calculated from dose-response experiments were 2.9 (Chaah), 2.1 (Lenggeng), 3.3 (Bidor), and 2.8 (Temerloh). Two point mutations were detected in position 875 within the 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) gene resistant population, causing a C to T transition leading to Pro106 to Ser106 substitution in Bidor and Temerloh or C to A transversion leading to Pro 106 to Thr106 substitution in Chaah. Sequence comparisons of the 3115 bp of the EPSPS gene revealed identical sequences in both the susceptible (S) and resistant (R) populations of Lenggeng. The identical sequences in both the Lenggeng populations, S and R, suggest that the resistance mechanism found in Lenggeng R may be different from Chaah R, Bidor R, and Temerloh R. The polymerase chain reaction (PCR) amplification of specific alleles (PASA) was developed to detect 2 distinct single-point mutations, probably conferring herbicide resistance in Chaah R, Bidor R, and Temerloh R. PASA profiles showed only one DNA fragment for the entire S population and 2 different additional fragments, each specific for one resistance allele, for the R population.

Original languageEnglish
Pages (from-to)407-414
Number of pages8
JournalAustralian Journal of Agricultural Research
Volume55
Issue number4
DOIs
Publication statusPublished - 2004

Fingerprint

glyphosate
Eleusine
Eleusine indica
polymerase chain reaction
Alleles
alleles
Polymerase Chain Reaction
Population
point mutation
Point Mutation
3-Phosphoshikimate 1-Carboxyvinyltransferase
Herbicide Resistance
herbicide resistance
Malaysia
resistance mechanisms
Genes
dose response
genes
Phosphates

Keywords

  • Dose response
  • EPSPS
  • Herbicide resistance
  • PCR amplification of specific alleles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "Glyphosate-resistant populations of Eleusine indica (L.) Gaertn. were found in 4 areas in Malaysia that had received repeated applications of glyphosate for a period ranging from 5 to 15 years. The resistance ratios calculated from dose-response experiments were 2.9 (Chaah), 2.1 (Lenggeng), 3.3 (Bidor), and 2.8 (Temerloh). Two point mutations were detected in position 875 within the 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) gene resistant population, causing a C to T transition leading to Pro106 to Ser106 substitution in Bidor and Temerloh or C to A transversion leading to Pro 106 to Thr106 substitution in Chaah. Sequence comparisons of the 3115 bp of the EPSPS gene revealed identical sequences in both the susceptible (S) and resistant (R) populations of Lenggeng. The identical sequences in both the Lenggeng populations, S and R, suggest that the resistance mechanism found in Lenggeng R may be different from Chaah R, Bidor R, and Temerloh R. The polymerase chain reaction (PCR) amplification of specific alleles (PASA) was developed to detect 2 distinct single-point mutations, probably conferring herbicide resistance in Chaah R, Bidor R, and Temerloh R. PASA profiles showed only one DNA fragment for the entire S population and 2 different additional fragments, each specific for one resistance allele, for the R population.",
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T1 - Glyphosate resistance in Eleusine indica (L.) Gaertn. from different origins and polymerase chain reaction amplification of specific alleles

AU - Ng, C. H.

AU - V Ratnam, R Wickneswari

AU - Salmijah, S.

AU - Teng, Y. T.

AU - Sahid, Ismail

PY - 2004

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N2 - Glyphosate-resistant populations of Eleusine indica (L.) Gaertn. were found in 4 areas in Malaysia that had received repeated applications of glyphosate for a period ranging from 5 to 15 years. The resistance ratios calculated from dose-response experiments were 2.9 (Chaah), 2.1 (Lenggeng), 3.3 (Bidor), and 2.8 (Temerloh). Two point mutations were detected in position 875 within the 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) gene resistant population, causing a C to T transition leading to Pro106 to Ser106 substitution in Bidor and Temerloh or C to A transversion leading to Pro 106 to Thr106 substitution in Chaah. Sequence comparisons of the 3115 bp of the EPSPS gene revealed identical sequences in both the susceptible (S) and resistant (R) populations of Lenggeng. The identical sequences in both the Lenggeng populations, S and R, suggest that the resistance mechanism found in Lenggeng R may be different from Chaah R, Bidor R, and Temerloh R. The polymerase chain reaction (PCR) amplification of specific alleles (PASA) was developed to detect 2 distinct single-point mutations, probably conferring herbicide resistance in Chaah R, Bidor R, and Temerloh R. PASA profiles showed only one DNA fragment for the entire S population and 2 different additional fragments, each specific for one resistance allele, for the R population.

AB - Glyphosate-resistant populations of Eleusine indica (L.) Gaertn. were found in 4 areas in Malaysia that had received repeated applications of glyphosate for a period ranging from 5 to 15 years. The resistance ratios calculated from dose-response experiments were 2.9 (Chaah), 2.1 (Lenggeng), 3.3 (Bidor), and 2.8 (Temerloh). Two point mutations were detected in position 875 within the 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) gene resistant population, causing a C to T transition leading to Pro106 to Ser106 substitution in Bidor and Temerloh or C to A transversion leading to Pro 106 to Thr106 substitution in Chaah. Sequence comparisons of the 3115 bp of the EPSPS gene revealed identical sequences in both the susceptible (S) and resistant (R) populations of Lenggeng. The identical sequences in both the Lenggeng populations, S and R, suggest that the resistance mechanism found in Lenggeng R may be different from Chaah R, Bidor R, and Temerloh R. The polymerase chain reaction (PCR) amplification of specific alleles (PASA) was developed to detect 2 distinct single-point mutations, probably conferring herbicide resistance in Chaah R, Bidor R, and Temerloh R. PASA profiles showed only one DNA fragment for the entire S population and 2 different additional fragments, each specific for one resistance allele, for the R population.

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