Glycogen synthase from rabbit skeletal muscle. Amino acid sequence at the sites phosphorylated by glycogen synthase kinase-3, and extension of the N-terminal sequence containing the site phosphorylated by phosphorylase kinase.

D. B. Rylatt, A. Aitken, T. Bilham, G. D. Condon, Mohammed Noor Embi, P. Cohen

Research output: Contribution to journalArticle

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Abstract

Glycogen synthase kinase-3 phosphorylates three serine residues on glycogen synthase (sites 3a, 3b and 3c) which are all located in the same nine-amino-acid segment of the polypeptide chain. The sequence in this region is: Arg-Tyr-Pro-Arg-Pro-Ala-Ser(P)-Val-Pro-Pro-Ser(P)-Pro-Ser-Leu-Ser(P)-Arg-. These serine residues are distinct from the sites phosphorylated preferentially by cyclic-AMP-dependent protein kinase (sites 1a and 1b) and phosphorylase kinase (site 2). The N-terminal sequence of glycogen synthase containing the serine residue phosphorylated by phosphorylase kinase has been extended. The sequence in this region is: Pro-Leu-Ser-Arg-Thr-Leu-Ser(P)-Val-Ser-Ser-Leu-Pro-Gly-Leu-Glu-Asp-Trp-Glu-Asp- Glu-Phe-Asp-Leu-Glu-Asn-Ser-Val-Leu-Phe-(Asx2,Glx2,Ala2,Val2,Lys)-. The similarity to the N-terminal sequence of phosphorylase is confined to the immediate vicinity of the phosphorylation site (residues 4--15). The relationship of glycogen synthase kinase-3 to glycogen synthase kinases that have been described by other laboratories is discussed.

Original languageEnglish
Pages (from-to)529-537
Number of pages9
JournalEuropean Journal of Biochemistry
Volume107
Issue number2
Publication statusPublished - Jun 1980
Externally publishedYes

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Phosphorylase Kinase
Glycogen Synthase Kinase 3
Glycogen Synthase
Serine
Muscle
Amino Acid Sequence
Skeletal Muscle
Rabbits
Amino Acids
Glycogen Synthase Kinases
Phosphorylases
Phosphorylation
Cyclic AMP-Dependent Protein Kinases
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glycogen synthase from rabbit skeletal muscle. Amino acid sequence at the sites phosphorylated by glycogen synthase kinase-3, and extension of the N-terminal sequence containing the site phosphorylated by phosphorylase kinase. / Rylatt, D. B.; Aitken, A.; Bilham, T.; Condon, G. D.; Embi, Mohammed Noor; Cohen, P.

In: European Journal of Biochemistry, Vol. 107, No. 2, 06.1980, p. 529-537.

Research output: Contribution to journalArticle

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AU - Condon, G. D.

AU - Embi, Mohammed Noor

AU - Cohen, P.

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