Giardia intestinalis genotypes

Risk factors and correlation with clinical symptoms

A. K. Mohammed Mahdy, Johari Surin, Kiew Lian Wan, A. Mohd-Adnan, M. S Hesham Al-Mekhlafi, Y. A L Lim

Research output: Contribution to journalArticle

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Abstract

This study was conducted to identify genotypes related risk factors of Giardia intestinalis in an Orang Asli (aboriginal) community in Pahang, Malaysia. Stool samples were collected from 321 individuals aged between 2 and 76 years old, of whom 160 were males and 161 were females. Faecal samples were processed with trichrome staining technique for the primary identification of G. intestinalis. Molecular identification was carried out by the amplification of a partial SSU rRNA gene using nested PCR. PCR products were purified and genotyped. 42 samples successfully amplified from the 76 positive faecal samples, only 1 was Assemblage A, the rest were Assemblage B. Risk analysis based on the detected genotypes of Giardia using univariate analysis and logistic regression identified three significant risk factors of giardiasis caused by assemblage B which included children ≤12 years (OR = 13.56, 95% CI = 1.79-102.64, p = 0.012), females (OR = 2.52, 95% CI = 1.11-5.75, p = 0.027) and eating fresh fruits (OR = 7.78, 95% CI = 1.01-60.00, p = 0.049). Assemblage B infection was significantly correlated with clinical symptoms of giardiasis (OR = 2.4, 95% CI = 1.13-5.12, p = 0.019). Females infected with Assemblage B were at higher risk of manifesting gastroenteritis signs and symptoms (OR = 3.9, 95% CI = 1.50-10.31, p = 0.004). It has been concluded that giardiasis is still a public health problem in Orang Asli community and most commonly caused by assemblage B. The dynamic of transmission is most probably anthroponotic which is human to human either directly or indirectly through contaminated food. This route of transmission should be considered in the control strategy of the disease. Mass treatment together with health education could be the most practical intervention for reducing the infection. Those at high risk should receive more attention from public health authorities.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalActa Tropica
Volume112
Issue number1
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Giardiasis
Giardia lamblia
giardiasis
signs and symptoms (animals and humans)
risk factors
Genotype
genotype
Public Health
Giardia
Polymerase Chain Reaction
public health
Malaysia
Gastroenteritis
Infection
rRNA Genes
Health Education
sampling
health education
Signs and Symptoms
risk analysis

Keywords

  • Clinical symptoms
  • Genotyping
  • Giardia intestinalis
  • Malaysia
  • Risk factors

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Mohammed Mahdy, A. K., Surin, J., Wan, K. L., Mohd-Adnan, A., Al-Mekhlafi, M. S. H., & Lim, Y. A. L. (2009). Giardia intestinalis genotypes: Risk factors and correlation with clinical symptoms. Acta Tropica, 112(1), 67-70. https://doi.org/10.1016/j.actatropica.2009.06.012

Giardia intestinalis genotypes : Risk factors and correlation with clinical symptoms. / Mohammed Mahdy, A. K.; Surin, Johari; Wan, Kiew Lian; Mohd-Adnan, A.; Al-Mekhlafi, M. S Hesham; Lim, Y. A L.

In: Acta Tropica, Vol. 112, No. 1, 10.2009, p. 67-70.

Research output: Contribution to journalArticle

Mohammed Mahdy, AK, Surin, J, Wan, KL, Mohd-Adnan, A, Al-Mekhlafi, MSH & Lim, YAL 2009, 'Giardia intestinalis genotypes: Risk factors and correlation with clinical symptoms', Acta Tropica, vol. 112, no. 1, pp. 67-70. https://doi.org/10.1016/j.actatropica.2009.06.012
Mohammed Mahdy, A. K. ; Surin, Johari ; Wan, Kiew Lian ; Mohd-Adnan, A. ; Al-Mekhlafi, M. S Hesham ; Lim, Y. A L. / Giardia intestinalis genotypes : Risk factors and correlation with clinical symptoms. In: Acta Tropica. 2009 ; Vol. 112, No. 1. pp. 67-70.
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