Genotoxicity following organophosphate pesticides exposure among orang asli children living in an agricultural island in kuala langat, Selangor, Malaysia

J. M. Sutris, V. How, S. A. Sumeri, M. Muhammad, D. Sardi, M. T. Mohd Mokhtar, H. Mohammad, H. F. Ghazi, Zaleha Md Isa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Agriculture is an important sector for the Malaysian economy. The use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. Children living near agricultural fields are at risk of pesticide poisoning. Objective: To evaluate the genotoxic risk among children who exposed to pesticides and measure DNA damage due to pesticides exposure. Methods: In a cross-sectional study 180 Orang Asli Mah Meri children aged between 7 and 12 years were studied. They were all living in an agricultural island in Kuala Langat, Selangor, Malaysia. The data for this study were collected via modified validated questionnaire and food frequency questionnaire, which consisted of 131 food items. 6 urinary organophosphate metabolites were used as biomarkers for pesticides exposure. For genotoxic risk or genetic damage assessment, the level of DNA damage from exfoliated buccal mucosa cells was measured using the comet assay electrophoresis method. Results: Out of 180 respondents, 84 (46.7%) showed positive traces of organophosphate metabolites in their urine. Children with detectable urinary pesticide had a longer tail length (median 43.5; IQR 30.9 to 68.1 µm) than those with undetectable urinary pesticides (median 24.7; IQR 9.5 to 48.1 µm). There was a significant association between the extent of DNA damage and the children's age, length of residence in the area, pesticides detection, and frequency of apple consumption. Conclusion: The organophosphate genotoxicity among children is associated with the amount of exposure (detectability of urinary pesticide) and length of residence in (exposure) the study area.

Original languageEnglish
Pages (from-to)42-51
Number of pages10
JournalInternational Journal of Occupational and Environmental Medicine
Volume7
Issue number1
Publication statusPublished - 1 Jan 2016

Fingerprint

Organophosphates
Malaysia
Pesticides
Islands
DNA Damage
Agriculture
Food
Comet Assay
Mouth Mucosa
Malus
Poisoning
Insects
Electrophoresis
Tail
Cross-Sectional Studies
Biomarkers
Urine

Keywords

  • Child
  • Comet assay
  • Malaysia
  • Mutagenicity tests
  • Organophosphate poisoning
  • Organophosphates
  • Pesticides

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Genotoxicity following organophosphate pesticides exposure among orang asli children living in an agricultural island in kuala langat, Selangor, Malaysia. / Sutris, J. M.; How, V.; Sumeri, S. A.; Muhammad, M.; Sardi, D.; Mohd Mokhtar, M. T.; Mohammad, H.; Ghazi, H. F.; Md Isa, Zaleha.

In: International Journal of Occupational and Environmental Medicine, Vol. 7, No. 1, 01.01.2016, p. 42-51.

Research output: Contribution to journalArticle

Sutris, J. M. ; How, V. ; Sumeri, S. A. ; Muhammad, M. ; Sardi, D. ; Mohd Mokhtar, M. T. ; Mohammad, H. ; Ghazi, H. F. ; Md Isa, Zaleha. / Genotoxicity following organophosphate pesticides exposure among orang asli children living in an agricultural island in kuala langat, Selangor, Malaysia. In: International Journal of Occupational and Environmental Medicine. 2016 ; Vol. 7, No. 1. pp. 42-51.
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AU - Muhammad, M.

AU - Sardi, D.

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