Genetic variation of Trigonobalanus verticillata, a primitive species of Fagaceae, in Malaysia revealed by chloroplast sequences and AFLP markers

Koichi Kamiya, Ko Harada, Mahani Mansor Clyde, Abdul Latiff Mohamed

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    The genetic variation of Trigonobalanus verticillata, the most recently described genus of Fagaceae, was studied using chloroplast DNA sequences and AFLP fingerprinting. This species has a restricted distribution that is known to include seven localities in tropical lower montane forests in Malaysia and Indonesia. A total of 75 individuals were collected from Bario, Kinabalu, and Fraser's Hill in Malaysia. The sequences of rbcL, marK, and three non-coding regions (atpB-rbcL spacer, trnL intron, and trnL-trnF spacer) were determined for 19 individuals from these populations. We found a total of 30 nucleotide substitutions and four length variations, which allowed identification of three haplotypes characterizing each population. No substitutions were detected within populations, while the tandem repeats in the trnL-trnF spacer had a variable repeat number of a 20-bp motif only in Kinabalu. The differentiation of the populations inferred from the cpDNA molecular clock calibrated with paleontological data was estimated to be 8.3 MYA between Bario and Kinabalu, and 16.7 MYA between Fraser's Hill and the other populations. In AFLP analysis, four selective primer pairs yielded a total of 431 loci, of which 340 (78.9%) were polymorphic. The results showed relatively high gene diversity (H S = 0.153 and H T = 0.198) and nucleotide diversity (π S = 0.0132 and π T = 0.0168) both within and among the populations. Although the cpDNA data suggest that little or no gene flow occurred between the populations via seeds, the fixation index estimated from AFLP data (F ST = 0.153 and N ST = 0.214) implies that some gene flow occurs between populations, possibly through pollen transfer.

    Original languageEnglish
    Pages (from-to)177-186
    Number of pages10
    JournalGenes and Genetic Systems
    Volume77
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Fagaceae
    Malaysia
    Chloroplasts
    amplified fragment length polymorphism
    chloroplasts
    Genes
    genetic variation
    Substitution reactions
    Nucleotides
    Population
    Chloroplast DNA
    Tandem Repeat Sequences
    DNA sequences
    chloroplast DNA
    Introns
    Gene Flow
    Seed
    Clocks
    gene flow
    nucleotides

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Genetics
    • Genetics(clinical)

    Cite this

    Genetic variation of Trigonobalanus verticillata, a primitive species of Fagaceae, in Malaysia revealed by chloroplast sequences and AFLP markers. / Kamiya, Koichi; Harada, Ko; Clyde, Mahani Mansor; Mohamed, Abdul Latiff.

    In: Genes and Genetic Systems, Vol. 77, No. 3, 2002, p. 177-186.

    Research output: Contribution to journalArticle

    Kamiya, Koichi ; Harada, Ko ; Clyde, Mahani Mansor ; Mohamed, Abdul Latiff. / Genetic variation of Trigonobalanus verticillata, a primitive species of Fagaceae, in Malaysia revealed by chloroplast sequences and AFLP markers. In: Genes and Genetic Systems. 2002 ; Vol. 77, No. 3. pp. 177-186.
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