Genetic variation of Dryobalanops aromatica Gaertn. F. (Dipterocarpaceae) in Peninsular Malaysia using microsatellite DNA markers

L. S. Lim, R Wickneswari V Ratnam, S. L. Lee, A. Latiff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nine microsatellite DNA markers, developed for Shorea curtisii (i.e. Shc01, Shc02, Shc03, Shc04, Shc07, Shc08, Shc09, Shc11 and Shc17), and one, developed for Dryobalanops lanceolata [DL(GT)202], were tested on D. aromatica. The primer developed for D. lanceolata showed no amplification in D. aromatica. Although all the primers designed for S. curtisii produced amplifications, primer Shc01 and Shc08 yielded many non-specific bands. The other seven codominant microsatellite DNA loci were used to analyse the genetic variation of D. aromatica from five populations in Peninsular Malaysia, namely Lenggor and Ulu Sedili (Johore), Lesong (Pahang), Kanching (Selangor) and Bukit Sai (Terengganu). The expected mean heterozygosity (Hc) was high (0.709) with values ranging from 0.684 (Lenggor) to 0.735 (Lesong). Most of the populations showed high and positive fixation indices indicating an excess of homozygotes. This implies a high level of inbreeding which may be caused by selfing or/and mating between closely related individuals. A gene diversity analysis showed that 93.3% of the observed genetic diversity was contained within populations and 6.7% (GST = 0.067) was due to differences among populations. The smallest genetic distance (D) was between Bukit Sai and Lesong (0.068) and the largest, between Ulu Sedili and Lesong (0.477). Mean gene flow (Nm) was high (2.94) with the highest value being between Lenggor and Bukit S ai (-6.98). The lowest gene flow occurred between Kanching and Ulu Sedili (1.95). The findings from this study imply that the four natural populations of D. aromatica in the east coast of Peninsular Malaysia may have been a single unfragmented population in the recent past. The results of this study also supports an earlier alternative hypothesis that the Kanching population in the west coast of Peninsular Malaysia originated from the east coast populations. This is supported by the dendrogram derived from the UPGMA cluster analysis of genetic distances in that the Kanching population is closely related to the Lesong and Bukit Sai populations from the east coast.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalForest Genetics
Volume9
Issue number2
Publication statusPublished - 2002

Fingerprint

Dryobalanops aromatica
Dipterocarpaceae
Malaysia
Genetic Markers
Microsatellite Repeats
genetic variation
microsatellite repeats
DNA
genetic markers
coast
Population
gene flow
amplification
autogamy
inbreeding
coasts
heterozygosity
Gene Flow
fixation
cluster analysis

Keywords

  • Dipterocarpaceae
  • Dryobalanops aromatica
  • Genetic differentiation
  • Genetic variation
  • Microsatellite
  • Natural population

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Forestry
  • Ecology
  • Environmental Science(all)

Cite this

Genetic variation of Dryobalanops aromatica Gaertn. F. (Dipterocarpaceae) in Peninsular Malaysia using microsatellite DNA markers. / Lim, L. S.; V Ratnam, R Wickneswari; Lee, S. L.; Latiff, A.

In: Forest Genetics, Vol. 9, No. 2, 2002, p. 125-136.

Research output: Contribution to journalArticle

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