Genetic structure and haplotype diversity of tachypleus gigas population along the west coast of peninsular malaysia-inferred through mtDNA at rich region sequence analysis

M. Rozihan, Endom Ismail

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4 Citations (Scopus)

Abstract

A detailed investigation was carried out to determine the genetic structure and haplotype diversity of Malaysian horseshoe crab (Tachypleus gigas [Muller, 1785]) distributed along the west coast of Peninsular Malaysia. Mitochondrial DNA (AT rich region = 369 bp) analysis showed that T. gigas had 13 haplotypes along the Malaysian west coast of which 4 were unique to Selangor samples while 3 were unique to lohor sample and 1 each were unique to other two stations respectively. Highest haplotype diversity (π) was observed among the Selangor samples (0.873±0.071) followed by Langkawi, lohor and Kedah samples with 0.833±0.222, 0.752±0.066 and 0.733±0.155 values, respectively. Over all haplotype diversity of T. gigas in west coast of Malaysia was observed to be 0.797±0.129. Pair wise haplotype frequency (F ST) value were statistically significant (p<0.05) for all the groups except for Langkawi/Kedah samples indicating higher gene flow (Lower haplotype diversity) among these two populations. Average nucleotide diversity (TI) was higher in Selangor samples (0.0083±0.001) followed by lohor (0.0063±0.0011) and it was almost similar in Langkawi (0.0045±0.0012) and Kedah (0.0040±0.0008) samples which indicated higher polymorphic sites in Selangor and lohor samples while it was lower in Langkawi and Kedah samples. In addition phylogenetic analysis clearly clustered T. gigas samples from T. tridentatus samples indicating good phylogenetic signals in mfDNA AT rich region. Overall, findings from this study have important implications for proper management and conservation of this living fossil along the west coast of Peninsular Malaysia.

Original languageEnglish
Pages (from-to)298-302
Number of pages5
JournalBiotechnology
Volume10
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

Horseshoe Crabs
Genetic Structures
Malaysia
Mitochondrial DNA
Haplotypes
Sequence Analysis
Coastal zones
AT Rich Sequence
Population
Conservation
Gene Flow
Nucleotides
Genes

Keywords

  • Genetic conservation
  • Haplotype diversity
  • mtDNA AT rich region
  • Nucleotide diversity
  • Tachypleus gigas

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Genetic structure and haplotype diversity of tachypleus gigas population along the west coast of peninsular malaysia-inferred through mtDNA at rich region sequence analysis",
abstract = "A detailed investigation was carried out to determine the genetic structure and haplotype diversity of Malaysian horseshoe crab (Tachypleus gigas [Muller, 1785]) distributed along the west coast of Peninsular Malaysia. Mitochondrial DNA (AT rich region = 369 bp) analysis showed that T. gigas had 13 haplotypes along the Malaysian west coast of which 4 were unique to Selangor samples while 3 were unique to lohor sample and 1 each were unique to other two stations respectively. Highest haplotype diversity (π) was observed among the Selangor samples (0.873±0.071) followed by Langkawi, lohor and Kedah samples with 0.833±0.222, 0.752±0.066 and 0.733±0.155 values, respectively. Over all haplotype diversity of T. gigas in west coast of Malaysia was observed to be 0.797±0.129. Pair wise haplotype frequency (F ST) value were statistically significant (p<0.05) for all the groups except for Langkawi/Kedah samples indicating higher gene flow (Lower haplotype diversity) among these two populations. Average nucleotide diversity (TI) was higher in Selangor samples (0.0083±0.001) followed by lohor (0.0063±0.0011) and it was almost similar in Langkawi (0.0045±0.0012) and Kedah (0.0040±0.0008) samples which indicated higher polymorphic sites in Selangor and lohor samples while it was lower in Langkawi and Kedah samples. In addition phylogenetic analysis clearly clustered T. gigas samples from T. tridentatus samples indicating good phylogenetic signals in mfDNA AT rich region. Overall, findings from this study have important implications for proper management and conservation of this living fossil along the west coast of Peninsular Malaysia.",
keywords = "Genetic conservation, Haplotype diversity, mtDNA AT rich region, Nucleotide diversity, Tachypleus gigas",
author = "M. Rozihan and Endom Ismail",
year = "2011",
doi = "10.3923/biotech.2011.298.302",
language = "English",
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T1 - Genetic structure and haplotype diversity of tachypleus gigas population along the west coast of peninsular malaysia-inferred through mtDNA at rich region sequence analysis

AU - Rozihan, M.

AU - Ismail, Endom

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N2 - A detailed investigation was carried out to determine the genetic structure and haplotype diversity of Malaysian horseshoe crab (Tachypleus gigas [Muller, 1785]) distributed along the west coast of Peninsular Malaysia. Mitochondrial DNA (AT rich region = 369 bp) analysis showed that T. gigas had 13 haplotypes along the Malaysian west coast of which 4 were unique to Selangor samples while 3 were unique to lohor sample and 1 each were unique to other two stations respectively. Highest haplotype diversity (π) was observed among the Selangor samples (0.873±0.071) followed by Langkawi, lohor and Kedah samples with 0.833±0.222, 0.752±0.066 and 0.733±0.155 values, respectively. Over all haplotype diversity of T. gigas in west coast of Malaysia was observed to be 0.797±0.129. Pair wise haplotype frequency (F ST) value were statistically significant (p<0.05) for all the groups except for Langkawi/Kedah samples indicating higher gene flow (Lower haplotype diversity) among these two populations. Average nucleotide diversity (TI) was higher in Selangor samples (0.0083±0.001) followed by lohor (0.0063±0.0011) and it was almost similar in Langkawi (0.0045±0.0012) and Kedah (0.0040±0.0008) samples which indicated higher polymorphic sites in Selangor and lohor samples while it was lower in Langkawi and Kedah samples. In addition phylogenetic analysis clearly clustered T. gigas samples from T. tridentatus samples indicating good phylogenetic signals in mfDNA AT rich region. Overall, findings from this study have important implications for proper management and conservation of this living fossil along the west coast of Peninsular Malaysia.

AB - A detailed investigation was carried out to determine the genetic structure and haplotype diversity of Malaysian horseshoe crab (Tachypleus gigas [Muller, 1785]) distributed along the west coast of Peninsular Malaysia. Mitochondrial DNA (AT rich region = 369 bp) analysis showed that T. gigas had 13 haplotypes along the Malaysian west coast of which 4 were unique to Selangor samples while 3 were unique to lohor sample and 1 each were unique to other two stations respectively. Highest haplotype diversity (π) was observed among the Selangor samples (0.873±0.071) followed by Langkawi, lohor and Kedah samples with 0.833±0.222, 0.752±0.066 and 0.733±0.155 values, respectively. Over all haplotype diversity of T. gigas in west coast of Malaysia was observed to be 0.797±0.129. Pair wise haplotype frequency (F ST) value were statistically significant (p<0.05) for all the groups except for Langkawi/Kedah samples indicating higher gene flow (Lower haplotype diversity) among these two populations. Average nucleotide diversity (TI) was higher in Selangor samples (0.0083±0.001) followed by lohor (0.0063±0.0011) and it was almost similar in Langkawi (0.0045±0.0012) and Kedah (0.0040±0.0008) samples which indicated higher polymorphic sites in Selangor and lohor samples while it was lower in Langkawi and Kedah samples. In addition phylogenetic analysis clearly clustered T. gigas samples from T. tridentatus samples indicating good phylogenetic signals in mfDNA AT rich region. Overall, findings from this study have important implications for proper management and conservation of this living fossil along the west coast of Peninsular Malaysia.

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KW - Nucleotide diversity

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