Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method

Omalsaad, A. K M Aminul Islam, Murshida Akhter Jahan, Zahira Yaakob, Mohamad Osman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction of genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and seven kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB) method. RAPD analysis using two chloroplast primers and one mitochondrial primer produced about 62 bands of different sizes. Most of the fragments were polymorphic where 2-3 fragments were monomorphic in each primer. Sixteen accessions were classified into two main groups A and B through dendogram generated using RAPD polymorphism. Five kenaf accessions were included in group A, whereas nine roselle accessions and two kenaf accessions were included in group B. A group was formed with a range of similarity coefficients of 0.5333 to 0.8780 having all roselle accessions. Bengkalis was different from Hibiscus sp but it was close to roselle accessions. Noonsoon was very close to roselle accessions having a range of jaccard similarity coefficients of 0.5000 to 0.6326. It was evident from jaccad similarity coefficient of 0.8780 that the roselle varieties UKMR 1 and UKMR 2 were genetically closer to each other. On the other hand, V36 and Thainung showed the highest similarity coefficient (0.7878) among kenaf accessions in group A. This study has successfully differentiated between two species by using chloroplast and mitochondrial markers which was due to high level of polymorphism. However, the chloroplast primers were more effective than M13 to study the genetic variation between roselle and kenaf.

Original languageEnglish
Pages (from-to)247-258
Number of pages12
JournalEmirates Journal of Food and Agriculture
Volume26
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Hibiscus
Hibiscus cannabinus
Hibiscus sabdariffa
kenaf
genetic relationships
Polymerase Chain Reaction
chloroplasts
methodology
Chloroplasts
genetic polymorphism
bromides
genomics

Keywords

  • DNA extraction
  • Genetic similarity
  • Phylogenetics
  • RAPD-PCR optimization

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Animal Science and Zoology
  • Food Science
  • Applied Microbiology and Biotechnology

Cite this

Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method. / Omalsaad; Islam, A. K M Aminul; Jahan, Murshida Akhter; Yaakob, Zahira; Osman, Mohamad.

In: Emirates Journal of Food and Agriculture, Vol. 26, No. 3, 2014, p. 247-258.

Research output: Contribution to journalArticle

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