Genetic diversity of nephelium ramboutan-ake leenh. Assessed using RAPD and ISSR markers

M. M. Clyde, P. C. Chew, Normah Mohd. Noor, V. Ramanatha Rao, I. Salma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Genetic variation among Nephelium ramboutan-ake accessions maintained in field genebanks at MARDI Serdang and at MARDI Kemaman was assessed using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Values of genetic diversity based on Shannon index and genetic relatedness based on Jaccard index of genetic similarity differed depending on the groups of accessions surveyed and markers used. For 24 accessions from MARDI Kemaman ISSRs detected less polymorphism (87.10%) compared to RAPDs (97.33%). Besides, RAPDs also revealed higher genetic diversity (H0 5.45) than ISSRs (H0 1.99) with mean Jaccard index of genetic similarity 0.408 (RAPDs) and 0.561 (ISSRs) respectively. This study illustrates how choice of DNA markers can result in different estimates of genetic diversity and values of relatedness in N. ramboutan-ake.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages171-182
Number of pages12
Volume665
Publication statusPublished - 2005

Publication series

NameActa Horticulturae
Volume665
ISSN (Print)05677572

Fingerprint

Nephelium
random amplified polymorphic DNA technique
microsatellite repeats
genetic variation
genetic relationships
genetic polymorphism
genetic markers

Keywords

  • DNA markers
  • Genetic relatedness
  • Genetic similarity
  • Jaccard index
  • Polymorphism
  • Shannon index

ASJC Scopus subject areas

  • Horticulture

Cite this

Clyde, M. M., Chew, P. C., Mohd. Noor, N., Rao, V. R., & Salma, I. (2005). Genetic diversity of nephelium ramboutan-ake leenh. Assessed using RAPD and ISSR markers. In Acta Horticulturae (Vol. 665, pp. 171-182). (Acta Horticulturae; Vol. 665).

Genetic diversity of nephelium ramboutan-ake leenh. Assessed using RAPD and ISSR markers. / Clyde, M. M.; Chew, P. C.; Mohd. Noor, Normah; Rao, V. Ramanatha; Salma, I.

Acta Horticulturae. Vol. 665 2005. p. 171-182 (Acta Horticulturae; Vol. 665).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Clyde, MM, Chew, PC, Mohd. Noor, N, Rao, VR & Salma, I 2005, Genetic diversity of nephelium ramboutan-ake leenh. Assessed using RAPD and ISSR markers. in Acta Horticulturae. vol. 665, Acta Horticulturae, vol. 665, pp. 171-182.
Clyde MM, Chew PC, Mohd. Noor N, Rao VR, Salma I. Genetic diversity of nephelium ramboutan-ake leenh. Assessed using RAPD and ISSR markers. In Acta Horticulturae. Vol. 665. 2005. p. 171-182. (Acta Horticulturae).
Clyde, M. M. ; Chew, P. C. ; Mohd. Noor, Normah ; Rao, V. Ramanatha ; Salma, I. / Genetic diversity of nephelium ramboutan-ake leenh. Assessed using RAPD and ISSR markers. Acta Horticulturae. Vol. 665 2005. pp. 171-182 (Acta Horticulturae).
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