Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells

M. S. Hasmah, A. R. Omar, Kiew Lian Wan, M. Hair-Bejo, I. Aini

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It has been shown that a chicken anemia virus (CAV) isolates which had undergone 60 passages in MSB-1 cells (SMSC-1/P60, 3-1/P60) acquired 33-66 nucleotide substitutions at the coding region resulting in 13-16 amino acid changes as compared to the CAV isolates passaged only 5 times in MSB-1 cells (SMSC-1 and 3-1) (Chowdhury et al., Arch. Virol. 148, 2437-2448, 2003). In this study we found that alow CAV (BL-5) and a high CAV passage (BL-5/P90) differed by only 15 nucleotide substitutions resulting in 11 amino acid changes. Phylogenetic analysis based on VP1 also revealed that both isolates were close to each other but not to other CAV isolates from Malaysia, namely SMSC-1 and 3-1.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalActa Virologica
Volume48
Issue number2
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Chicken anemia virus
Cell Culture Techniques
Nucleotides
Amino Acids
Malaysia

Keywords

  • Amino acid substitutions
  • Chicken anemia virus
  • Coding regions
  • Nucleotide sequence
  • Nucleotide substitutions
  • Phylogenetic analysis
  • Virus passaging

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Hasmah, M. S., Omar, A. R., Wan, K. L., Hair-Bejo, M., & Aini, I. (2004). Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells. Acta Virologica, 48(2), 85-89.

Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells. / Hasmah, M. S.; Omar, A. R.; Wan, Kiew Lian; Hair-Bejo, M.; Aini, I.

In: Acta Virologica, Vol. 48, No. 2, 2004, p. 85-89.

Research output: Contribution to journalArticle

Hasmah, MS, Omar, AR, Wan, KL, Hair-Bejo, M & Aini, I 2004, 'Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells', Acta Virologica, vol. 48, no. 2, pp. 85-89.
Hasmah, M. S. ; Omar, A. R. ; Wan, Kiew Lian ; Hair-Bejo, M. ; Aini, I. / Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells. In: Acta Virologica. 2004 ; Vol. 48, No. 2. pp. 85-89.
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