Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase i (COI) and D-loop region sequences

Mohamad Azam Akmal Abu Bakar, Jeffrine Japning Rovie-Ryan, Ahmad Ampeng, Salmah Yaakop, Shukor Md. Nor, Badrul Munir Md. Zain

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Mousedeer is one of the primitive mammals that can be found mainly in Southeast-Asia region. There are two species of mousedeer in Malaysia which are Tragulus kanchil and Tragulus napu. Both species can be distinguish by size, coat coloration, and throat pattern but clear diagnosis still cannot be found. The objective of the study is to show the genetic distance relationship between T. kanchil and T. napu and their population based on mitochondrial DNA (mtDNA) cytochrome oxidase I (COI) and D-loop region. There are 42 sample of mousedeer were used in this study collected by PERHILITAN from different locality. Another 29 D-loop sequence were retrieved from Genbank for comparative analysis. All sample were amplified using universal primer and species-specific primer for COI and D-loop genes via PCR process. The amplified sequences were analyzed to determine genetic distance of T. kanchil and T. napu. From the analysis, the average genetic distance between T. kanchil and T. napu based on locus COI and D-loop were 0.145 and 0.128 respectively. The genetic distance between populations of T. kanchil based on locus COI was between 0.003-0.013. For locus D-loop, genetic distance analysis showed distance in relationship between west-coast populations to east-coast population of T. kanchil. COI and D-loop mtDNA region provided a clear picture on the relationship within the mousedeer species. Last but not least, conservation effort toward protecting this species can be done by study the molecular genetics and prevent the extinction of this species.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

oxidase
cytochromes
deoxyribonucleic acid
loci
primers
coasts
Southeast Asia
Malaysia
mammals
throats
genes
conservation
extinction
color

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Bakar, M. A. A. A., Rovie-Ryan, J. J., Ampeng, A., Yaakop, S., Md. Nor, S., & Md. Zain, B. M. (2018). Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase i (COI) and D-loop region sequences. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020035] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027950

Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase i (COI) and D-loop region sequences. / Bakar, Mohamad Azam Akmal Abu; Rovie-Ryan, Jeffrine Japning; Ampeng, Ahmad; Yaakop, Salmah; Md. Nor, Shukor; Md. Zain, Badrul Munir.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020035.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bakar, MAAA, Rovie-Ryan, JJ, Ampeng, A, Yaakop, S, Md. Nor, S & Md. Zain, BM 2018, Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase i (COI) and D-loop region sequences. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020035, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027950
Bakar MAAA, Rovie-Ryan JJ, Ampeng A, Yaakop S, Md. Nor S, Md. Zain BM. Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase i (COI) and D-loop region sequences. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020035 https://doi.org/10.1063/1.5027950
Bakar, Mohamad Azam Akmal Abu ; Rovie-Ryan, Jeffrine Japning ; Ampeng, Ahmad ; Yaakop, Salmah ; Md. Nor, Shukor ; Md. Zain, Badrul Munir. / Genetic distance of Malaysian mousedeer based on mitochondrial DNA cytochrome oxidase i (COI) and D-loop region sequences. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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