Genetic and antigenic variance of foot-and-mouth disease virus type Asia1

O. Marquardt, Md. Mostafizur Rahman, B. Freiberg

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The capsid protein encoding genes of five recent type Asia1 foot-and-mouth disease virus isolates, representative of three genotypes, were sequenced. The deduced amino acid sequences were aligned to each other and to two published sequences. The sequence differences suggested different antigenic properties of the isolates. One isolate was used to generate monoclonal antibodies (mAbs) which were analyzed for neutralizing activity and reactivity with trypsinized virus. Trypsin removes the major antigenic sites located at VP1. The five virus isolates formed three reaction patterns with the mAbs, irrespective of their genotype. Combination of all data allowed to suggest the location of the epitope of each antibody: the VP1 G-H and the VP2 B-C loop, the VP3 B-B knob, and the N-terminus of VP2, respectively, were involved.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalArchives of Virology
Volume145
Issue number1
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Foot-and-Mouth Disease Virus
Genotype
Monoclonal Antibodies
Viruses
Capsid Proteins
Trypsin
Epitopes
Amino Acid Sequence
Antibodies
Genes

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Genetic and antigenic variance of foot-and-mouth disease virus type Asia1. / Marquardt, O.; Rahman, Md. Mostafizur; Freiberg, B.

In: Archives of Virology, Vol. 145, No. 1, 2000, p. 149-157.

Research output: Contribution to journalArticle

@article{2837652e550b460684b26b0c529b2dc1,
title = "Genetic and antigenic variance of foot-and-mouth disease virus type Asia1",
abstract = "The capsid protein encoding genes of five recent type Asia1 foot-and-mouth disease virus isolates, representative of three genotypes, were sequenced. The deduced amino acid sequences were aligned to each other and to two published sequences. The sequence differences suggested different antigenic properties of the isolates. One isolate was used to generate monoclonal antibodies (mAbs) which were analyzed for neutralizing activity and reactivity with trypsinized virus. Trypsin removes the major antigenic sites located at VP1. The five virus isolates formed three reaction patterns with the mAbs, irrespective of their genotype. Combination of all data allowed to suggest the location of the epitope of each antibody: the VP1 G-H and the VP2 B-C loop, the VP3 B-B knob, and the N-terminus of VP2, respectively, were involved.",
author = "O. Marquardt and Rahman, {Md. Mostafizur} and B. Freiberg",
year = "2000",
language = "English",
volume = "145",
pages = "149--157",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "1",

}

TY - JOUR

T1 - Genetic and antigenic variance of foot-and-mouth disease virus type Asia1

AU - Marquardt, O.

AU - Rahman, Md. Mostafizur

AU - Freiberg, B.

PY - 2000

Y1 - 2000

N2 - The capsid protein encoding genes of five recent type Asia1 foot-and-mouth disease virus isolates, representative of three genotypes, were sequenced. The deduced amino acid sequences were aligned to each other and to two published sequences. The sequence differences suggested different antigenic properties of the isolates. One isolate was used to generate monoclonal antibodies (mAbs) which were analyzed for neutralizing activity and reactivity with trypsinized virus. Trypsin removes the major antigenic sites located at VP1. The five virus isolates formed three reaction patterns with the mAbs, irrespective of their genotype. Combination of all data allowed to suggest the location of the epitope of each antibody: the VP1 G-H and the VP2 B-C loop, the VP3 B-B knob, and the N-terminus of VP2, respectively, were involved.

AB - The capsid protein encoding genes of five recent type Asia1 foot-and-mouth disease virus isolates, representative of three genotypes, were sequenced. The deduced amino acid sequences were aligned to each other and to two published sequences. The sequence differences suggested different antigenic properties of the isolates. One isolate was used to generate monoclonal antibodies (mAbs) which were analyzed for neutralizing activity and reactivity with trypsinized virus. Trypsin removes the major antigenic sites located at VP1. The five virus isolates formed three reaction patterns with the mAbs, irrespective of their genotype. Combination of all data allowed to suggest the location of the epitope of each antibody: the VP1 G-H and the VP2 B-C loop, the VP3 B-B knob, and the N-terminus of VP2, respectively, were involved.

UR - http://www.scopus.com/inward/record.url?scp=0033952031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033952031&partnerID=8YFLogxK

M3 - Article

VL - 145

SP - 149

EP - 157

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 1

ER -