Generation of dendritic cells from acute myeloid leukaemia cells and monocytes

our local experience.

M. N. Lim, Leong Chooi Fun, S. K. Cheong, H. F. Seow

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dendritic cells (DC) are efficient and potent antigen-presenting cells. Pilot clinical trials indicated that DC loaded with tumour antigen could induce tumour-specific immune responses in various cancers including B-cell lymphoma, melanoma and prostate cancer. Owing to extensively low number of DC in the blood circulation, a variety of sources have been used to generate DC including monocytes, CD34+ stem cells and even with leukaemic blast cells. We demonstrate here a simple method to generate DC from acute myeloid leukaemia (AML) cells and monocytes from healthy donor or remission samples. AML cells or monocytes were cultured in RPMI 1640 media supplemented with foetal bovine serum or autologous serum where possible and different combinations of cytokines GM-CSF, IL-4 and TNF-alpha. The generated DC were evaluated for their morphology by phase contrast microscopy and May Grunwald Giemsa staining. Viability of cells was determined by trypan blue dye exclusion. Percentage of yields and immunophenotypes were carried out by flow cytometry. We found that cultured AML cells and monocytes developed morphological and immuno-phenotypic characteristics of DC. Monocytes are better than AML blast in generating DC and serve as a ready source for dendritic cell vaccine development.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalThe Malaysian journal of pathology
Volume25
Issue number2
Publication statusPublished - 2003

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Myeloid Cells
Acute Myeloid Leukemia
Dendritic Cells
Monocytes
Phase-Contrast Microscopy
Trypan Blue
Blood Circulation
B-Cell Lymphoma
Neoplasm Antigens
Antigen-Presenting Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Serum
Interleukin-4
Melanoma
Neoplasms
Prostatic Neoplasms
Cell Survival
Flow Cytometry
Coloring Agents
Stem Cells

Cite this

Generation of dendritic cells from acute myeloid leukaemia cells and monocytes : our local experience. / Lim, M. N.; Chooi Fun, Leong; Cheong, S. K.; Seow, H. F.

In: The Malaysian journal of pathology, Vol. 25, No. 2, 2003, p. 107-112.

Research output: Contribution to journalArticle

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