Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro

P. F. Choong, P. L. Mok, S. K. Cheong, Leong Chooi Fun, K. Y. Then

Research output: Contribution to journalArticle

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Abstract

Background: The multipotency of stromal cells has been studied extensively. It has been reported that mesenchymal stromal cells (MSC) are capable of differentiating into cells of multilineage. Different methods and reagents have been used to induce the differentiation of MSC. We investigated the efficacy of different growth factors in inducing MSC differentiation into neurons. Methods: MSC from human BM were isolated and cultured in media supplemented with 10% FBS. These cells were identified and later induced to differentiate into neuron-like cells using different neurotrophic factors. Three different growth factors were used, either alone or in combination: brain-derived neurotrophic factor, epidermal growth factor and neural growth factor. Results: After 10 days of culture, MSC showed neuron-like morphologic changes. Immunostaining showed that these cells expressed markers for neurons (growth-associated protein-43, neuron-specific nuclear protein and neurofilament 200 kDa) and expression of these markers suggested the transition of immature stages to more mature stages of neuron-like cells. Discussion: Our results show that BM-derived MSC can differentiate not only into target cells of mesodermal origin but also neuron-like cells of ectodermal origin. The findings show that a combination of growth factors is more effective in inducing MSC into neuron-like cells.

Original languageEnglish
Pages (from-to)170-183
Number of pages14
JournalCytotherapy
Volume9
Issue number2
DOIs
Publication statusPublished - 2007

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Mesenchymal Stromal Cells
Neurons
Intercellular Signaling Peptides and Proteins
GAP-43 Protein
In Vitro Techniques
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Stromal Cells
Nuclear Proteins
Epidermal Growth Factor
Cell Differentiation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro. / Choong, P. F.; Mok, P. L.; Cheong, S. K.; Chooi Fun, Leong; Then, K. Y.

In: Cytotherapy, Vol. 9, No. 2, 2007, p. 170-183.

Research output: Contribution to journalArticle

Choong, P. F. ; Mok, P. L. ; Cheong, S. K. ; Chooi Fun, Leong ; Then, K. Y. / Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro. In: Cytotherapy. 2007 ; Vol. 9, No. 2. pp. 170-183.
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