Gene-Specific Repair of 6-4 Photoproducts in Trichothiodystrophy Cells

Sheila Nathan, Anneke Van Hoffen, Leon H F Mullenders, Lynne V. Mayne

Research output: Contribution to journalArticle

Abstract

TTD1BI cells are non-hypersensitive to UV irradiation and perform normal genome repair of pyrimidine dimers but fail to excise 6-4 photoproducts and, concomitantly, are unable to restore RNA synthesis levels following UV irradiation. This pointed to a defect in gene-specific repair and this study was undertaken to examine repair of 6-4 photoproducts at the gene-level. The results indicated a defect in gene-specific repair of 6-4 photoproducts in active genes, although strand-specificity of 6-4 photoproduct removal was essentially similar to that of normal cells. These findings indicate that the near normal UV resistance of TTD1BI cells may be due to the inability of these cells to remove DNA lesions preferentially, as well as to the cells opting out of the cell cycle to repair damage before resuming replication.

Original languageEnglish
Pages (from-to)554-560
Number of pages7
JournalJournal of Biochemistry and Molecular Biology
Volume32
Issue number6
Publication statusPublished - 30 Nov 1999

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Trichothiodystrophy Syndromes
Repair
Genes
Irradiation
Pyrimidine Dimers
Defects
Cell Cycle
Cells
Genome
RNA
DNA

Keywords

  • DNA repair
  • Gene-specific repair
  • Trichothiodystrophy
  • UV sensitivity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Gene-Specific Repair of 6-4 Photoproducts in Trichothiodystrophy Cells. / Nathan, Sheila; Van Hoffen, Anneke; Mullenders, Leon H F; Mayne, Lynne V.

In: Journal of Biochemistry and Molecular Biology, Vol. 32, No. 6, 30.11.1999, p. 554-560.

Research output: Contribution to journalArticle

Nathan, S, Van Hoffen, A, Mullenders, LHF & Mayne, LV 1999, 'Gene-Specific Repair of 6-4 Photoproducts in Trichothiodystrophy Cells', Journal of Biochemistry and Molecular Biology, vol. 32, no. 6, pp. 554-560.
Nathan, Sheila ; Van Hoffen, Anneke ; Mullenders, Leon H F ; Mayne, Lynne V. / Gene-Specific Repair of 6-4 Photoproducts in Trichothiodystrophy Cells. In: Journal of Biochemistry and Molecular Biology. 1999 ; Vol. 32, No. 6. pp. 554-560.
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