Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia

C. H. Ng, R Wickneswari V Ratnam, S. Salmijah, Y. T. Teng, Ismail Sahid

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86 Citations (Scopus)

Abstract

Resistant (R) and susceptible (S) biotypes of Eleusine indica were collected from four areas, namely Chaah, Lenggeng, Bidor and Temerloh, in Malaysia. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR)-RFLP analyses using SphI restriction enzyme were able to differentiate the R biotype from the S biotype by showing R-specific and S-specific polymorphisms in E. indica from three of the areas, with the exception of Temerloh where no polymorphisms were detected. The different DNA profiles for the R biotypes obtained indicate that SphI is not a useful diagnostic marker. The DNA polymorphisms detected in the 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase gene suggest that there are different mutation events leading to development of resistance to glyphosate. Partial sequencing of the EPSP synthase gene confirmed different mutations occurring with substitution of proline with serine or threonine at amino acid 106 for the R biotype in Chaah, Bidor and Temerloh.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalWeed Research
Volume43
Issue number2
DOIs
Publication statusPublished - Apr 2003

Fingerprint

Eleusine indica
biotype
glyphosate
biotypes
Malaysia
polymorphism
genetic polymorphism
gene
genes
mutation
restriction fragment length polymorphism
phosphate
DNA
threonine
serine
polymerase chain reaction
proline
substitution
amino acid
phosphates

Keywords

  • Eleusine indica
  • EPSP synthase
  • Herbicide resistance
  • PCR-RFLP
  • RFLP

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. / Ng, C. H.; V Ratnam, R Wickneswari; Salmijah, S.; Teng, Y. T.; Sahid, Ismail.

In: Weed Research, Vol. 43, No. 2, 04.2003, p. 108-115.

Research output: Contribution to journalArticle

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