Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells

Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain -Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun

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1 Citation (Scopus)

Abstract

SSalted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang, Malaysia. Balik Pulau was chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was found that the aqueous and methanol extracts of salted fish were not cytotoxic towards V79 lung fibroblast cells. It was also found that the extracts of salted fish from Balik Pulau were not mutagenic towards hprt gene of V79 lung fibroblast cells as the mutation frequency of the extracts did not exceed 3 times of the value for negative control mutation frequency. In conclusion, both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other toxicological profile could be assessed to determine the mechanism of toxicity of salted fish.

Original languageEnglish
Pages (from-to)1599-1603
Number of pages5
JournalSains Malaysiana
Volume42
Issue number11
Publication statusPublished - Nov 2013

Fingerprint

Malaysia
Methanol
Fishes
Fibroblasts
Lung
Mutation
Genes
Mutation Rate
Nasopharyngeal Neoplasms
Fisheries
Cooking
Toxicology

Keywords

  • Cytotoxic
  • Gene mutation
  • Hprt
  • Pulau Pinang
  • Salted fish

ASJC Scopus subject areas

  • General

Cite this

Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells. / Ghazali, Ahmad Rohi; Halim, Rasyidah Mohamad; Rajab, Nor Fadilah; -Ramli, Noorain; Abdullah, Rozaini; Kamarulzaman, Firdaus; Harun, Zaliha.

In: Sains Malaysiana, Vol. 42, No. 11, 11.2013, p. 1599-1603.

Research output: Contribution to journalArticle

Ghazali, Ahmad Rohi ; Halim, Rasyidah Mohamad ; Rajab, Nor Fadilah ; -Ramli, Noorain ; Abdullah, Rozaini ; Kamarulzaman, Firdaus ; Harun, Zaliha. / Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells. In: Sains Malaysiana. 2013 ; Vol. 42, No. 11. pp. 1599-1603.
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