Gene expression profiling in ethnic Malays with type 2 diabetes mellitus, with and without diabetic nephropathy

Faradianna E. Lokman, Norhashimah A. Seman, Azizal Safi Ismail, Nor Azwany Yaacob, Norlaila Mustafa, Amir S M Khir, Zanariah Hussein, Wan Nazaimoon Wan Mohamud

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Diabetic nephropathy (DN) is the most common cause of end-stage renal disease (ESRD) among type 2 diabetes mellitus patients (DM) in Malaysia. This study used microarray analysis to determine the gene expression profiling in ethnic Malay patients with type 2 DM. Methods: A total of 312 patients were recruited; 25 were on dialysis due to ESRD, 128 were classified as normoalbuminuric, 93 as microalbuminuric and 66 as macroalbuminuric, based on urine albumin to creatinine ratio of <3.5, between 3.5 and 35 and ≥35 mg/ mmol, respectively. Results: Microalbuminuria was associated with upand down-regulation of 2,694 and 2,538 genes, respectively, while macroalbuminuria was associated with up-regulation of 2,520 genes and down-regulation of 2,920 genes. There was significant up-regulation of 1,135 genes and down-regulation of 908 genes in the ESRD samples. Thirty-seven significantly upregulated genes and 40 down-regulated genes were commonly expressed in all 3 groups of patients with worsening of renal functions. Up-regulated genes included major histocompatibility complex (HLA-C), complement component 3a receptor 1 (C3AR1), solute carrier family 16, member 3 (SLC16A3) and solute car-rier family 9 (sodium/hydrogen exchanger) (SLC9A8). Consistently down-regulated genes included were bone morphogenetic phosphatase kinase (BMP2K), solute carrier family 12, member 1 (SLC12A1), solute carrier family 7 (SLC7A2), paternally expressed 10 (PEG10) and protein phosphatase 1 regulatory (inhibitor unit) (PPP1R1C). Conclusion: This study has identified several genes of interest, such as HLA-C, SLC16A3, SLC9A8, SLC12A1 and SLC7A2, that require verification of their roles as susceptibility genes for diabetic nephropathy in ethnic Malays with type 2 DM.

Original languageEnglish
Pages (from-to)778-789
Number of pages12
JournalJournal of Nephrology
Volume24
Issue number6
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Diabetic Nephropathies
Gene Expression Profiling
Type 2 Diabetes Mellitus
Genes
HLA-C Antigens
Chronic Kidney Failure
Down-Regulation
Member 1 Solute Carrier Family 12
Up-Regulation
Complement C3a
Sodium-Hydrogen Antiporter
Malaysia
Microarray Analysis
Major Histocompatibility Complex
Phosphoric Monoester Hydrolases
Dialysis
Albumins
Creatinine
Phosphotransferases
Urine

Keywords

  • Diabetic nephropathy
  • Gene expression
  • Malay
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Nephrology

Cite this

Lokman, F. E., Seman, N. A., Ismail, A. S., Yaacob, N. A., Mustafa, N., Khir, A. S. M., ... Mohamud, W. N. W. (2011). Gene expression profiling in ethnic Malays with type 2 diabetes mellitus, with and without diabetic nephropathy. Journal of Nephrology, 24(6), 778-789. https://doi.org/10.5301/JN.2011.6382

Gene expression profiling in ethnic Malays with type 2 diabetes mellitus, with and without diabetic nephropathy. / Lokman, Faradianna E.; Seman, Norhashimah A.; Ismail, Azizal Safi; Yaacob, Nor Azwany; Mustafa, Norlaila; Khir, Amir S M; Hussein, Zanariah; Mohamud, Wan Nazaimoon Wan.

In: Journal of Nephrology, Vol. 24, No. 6, 11.2011, p. 778-789.

Research output: Contribution to journalArticle

Lokman, FE, Seman, NA, Ismail, AS, Yaacob, NA, Mustafa, N, Khir, ASM, Hussein, Z & Mohamud, WNW 2011, 'Gene expression profiling in ethnic Malays with type 2 diabetes mellitus, with and without diabetic nephropathy', Journal of Nephrology, vol. 24, no. 6, pp. 778-789. https://doi.org/10.5301/JN.2011.6382
Lokman, Faradianna E. ; Seman, Norhashimah A. ; Ismail, Azizal Safi ; Yaacob, Nor Azwany ; Mustafa, Norlaila ; Khir, Amir S M ; Hussein, Zanariah ; Mohamud, Wan Nazaimoon Wan. / Gene expression profiling in ethnic Malays with type 2 diabetes mellitus, with and without diabetic nephropathy. In: Journal of Nephrology. 2011 ; Vol. 24, No. 6. pp. 778-789.
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abstract = "Background: Diabetic nephropathy (DN) is the most common cause of end-stage renal disease (ESRD) among type 2 diabetes mellitus patients (DM) in Malaysia. This study used microarray analysis to determine the gene expression profiling in ethnic Malay patients with type 2 DM. Methods: A total of 312 patients were recruited; 25 were on dialysis due to ESRD, 128 were classified as normoalbuminuric, 93 as microalbuminuric and 66 as macroalbuminuric, based on urine albumin to creatinine ratio of <3.5, between 3.5 and 35 and ≥35 mg/ mmol, respectively. Results: Microalbuminuria was associated with upand down-regulation of 2,694 and 2,538 genes, respectively, while macroalbuminuria was associated with up-regulation of 2,520 genes and down-regulation of 2,920 genes. There was significant up-regulation of 1,135 genes and down-regulation of 908 genes in the ESRD samples. Thirty-seven significantly upregulated genes and 40 down-regulated genes were commonly expressed in all 3 groups of patients with worsening of renal functions. Up-regulated genes included major histocompatibility complex (HLA-C), complement component 3a receptor 1 (C3AR1), solute carrier family 16, member 3 (SLC16A3) and solute car-rier family 9 (sodium/hydrogen exchanger) (SLC9A8). Consistently down-regulated genes included were bone morphogenetic phosphatase kinase (BMP2K), solute carrier family 12, member 1 (SLC12A1), solute carrier family 7 (SLC7A2), paternally expressed 10 (PEG10) and protein phosphatase 1 regulatory (inhibitor unit) (PPP1R1C). Conclusion: This study has identified several genes of interest, such as HLA-C, SLC16A3, SLC9A8, SLC12A1 and SLC7A2, that require verification of their roles as susceptibility genes for diabetic nephropathy in ethnic Malays with type 2 DM.",
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AB - Background: Diabetic nephropathy (DN) is the most common cause of end-stage renal disease (ESRD) among type 2 diabetes mellitus patients (DM) in Malaysia. This study used microarray analysis to determine the gene expression profiling in ethnic Malay patients with type 2 DM. Methods: A total of 312 patients were recruited; 25 were on dialysis due to ESRD, 128 were classified as normoalbuminuric, 93 as microalbuminuric and 66 as macroalbuminuric, based on urine albumin to creatinine ratio of <3.5, between 3.5 and 35 and ≥35 mg/ mmol, respectively. Results: Microalbuminuria was associated with upand down-regulation of 2,694 and 2,538 genes, respectively, while macroalbuminuria was associated with up-regulation of 2,520 genes and down-regulation of 2,920 genes. There was significant up-regulation of 1,135 genes and down-regulation of 908 genes in the ESRD samples. Thirty-seven significantly upregulated genes and 40 down-regulated genes were commonly expressed in all 3 groups of patients with worsening of renal functions. Up-regulated genes included major histocompatibility complex (HLA-C), complement component 3a receptor 1 (C3AR1), solute carrier family 16, member 3 (SLC16A3) and solute car-rier family 9 (sodium/hydrogen exchanger) (SLC9A8). Consistently down-regulated genes included were bone morphogenetic phosphatase kinase (BMP2K), solute carrier family 12, member 1 (SLC12A1), solute carrier family 7 (SLC7A2), paternally expressed 10 (PEG10) and protein phosphatase 1 regulatory (inhibitor unit) (PPP1R1C). Conclusion: This study has identified several genes of interest, such as HLA-C, SLC16A3, SLC9A8, SLC12A1 and SLC7A2, that require verification of their roles as susceptibility genes for diabetic nephropathy in ethnic Malays with type 2 DM.

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