Gene expression patterns distinguish breast carcinomas from normal breast tissues: The Malaysian context

Ivyna Bong Pau Ni, Zubaidah Zakaria, Rohaizak Muhammad, Norlia Abdullah, Naqiyah Ibrahim, Nor Aina Emran, Nor Hisham Abdullah, Sharifah Noor Akmal Syed Hussain

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Genomic and transcriptomic alterations that affect cellular processes, such as cell proliferation, differentiation, apoptosis and invasion, commonly occur in breast oncogenesis. Epidemiological evidence has proven that the risk of breast cancer predisposition varies among different ethnicities. This study aims to identify the transcriptome changes that commonly occur during the transition of normal breast epithelium to carcinoma in three local ethnic groups (Malays, Chinese and Indians). The gene expression patterns of 43 breast carcinomas with 43 patient-matched normal breast tissues were investigated using Affymetrix U133A GeneChip® (containing 22,283 probe sets targeting ~18,400 different transcripts) and analyzed with GeneSpring® GX10. Our findings revealed a total of 33 significantly differentially expressed genes, which showed>2-fold change at a 99.9% confidence interval level (p<0.001). The significantly differentially expressed genes included CD24, CD36, CD9, TACSTD1, TACSTD2, HBB, LEP, LPL, AKR1C1, AKR1C2 and AKR1C3. Our results indicate that the vast majority of gene expression changes, from normal breast epithelial to carcinoma, found in our three major ethnic populations are similar to those in the Caucasian population. Further study of the differentially expressed genes identified in our present study is needed to search for potential breast tumor biomarkers. This will eventually help to improve the therapeutic and treatment strategies for breast cancer patients in the future.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalPathology Research and Practice
Volume206
Issue number4
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Breast
Breast Neoplasms
Gene Expression
Genes
Carcinoma
Tumor Biomarkers
Transcriptome
Ethnic Groups
Population
Cell Differentiation
Carcinogenesis
Epithelium
Cell Proliferation
Confidence Intervals
Apoptosis
Therapeutics

Keywords

  • Breast carcinomas
  • Differentially expressed genes
  • Gene expression

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

Gene expression patterns distinguish breast carcinomas from normal breast tissues : The Malaysian context. / Pau Ni, Ivyna Bong; Zakaria, Zubaidah; Muhammad, Rohaizak; Abdullah, Norlia; Ibrahim, Naqiyah; Aina Emran, Nor; Hisham Abdullah, Nor; Syed Hussain, Sharifah Noor Akmal.

In: Pathology Research and Practice, Vol. 206, No. 4, 04.2010, p. 223-228.

Research output: Contribution to journalArticle

Pau Ni, Ivyna Bong ; Zakaria, Zubaidah ; Muhammad, Rohaizak ; Abdullah, Norlia ; Ibrahim, Naqiyah ; Aina Emran, Nor ; Hisham Abdullah, Nor ; Syed Hussain, Sharifah Noor Akmal. / Gene expression patterns distinguish breast carcinomas from normal breast tissues : The Malaysian context. In: Pathology Research and Practice. 2010 ; Vol. 206, No. 4. pp. 223-228.
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