Abstract
Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2-induced cell death is via neutralization of reactive oxygen species.
| Original language | English |
|---|---|
| Pages (from-to) | 373-384 |
| Number of pages | 12 |
| Journal | Integrative Cancer Therapies |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Sep 2017 |
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Keywords
- antioxidant
- cervical cancer
- flavokawain B
- HeLa
- iron sequestration
ASJC Scopus subject areas
- Oncology
- Complementary and alternative medicine
Cite this
Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells. / Yeap, Swee Keong; Abu, Nadiah; Akthar, Nadeem; Ho, Wan Yong; Ky, Huynh; Tan, Sheau Wei; Alitheen, Noorjahan Banu; Kamarul, Tunku.
In: Integrative Cancer Therapies, Vol. 16, No. 3, 01.09.2017, p. 373-384.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells
AU - Yeap, Swee Keong
AU - Abu, Nadiah
AU - Akthar, Nadeem
AU - Ho, Wan Yong
AU - Ky, Huynh
AU - Tan, Sheau Wei
AU - Alitheen, Noorjahan Banu
AU - Kamarul, Tunku
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2-induced cell death is via neutralization of reactive oxygen species.
AB - Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2-induced cell death is via neutralization of reactive oxygen species.
KW - antioxidant
KW - cervical cancer
KW - flavokawain B
KW - HeLa
KW - iron sequestration
UR - http://www.scopus.com/inward/record.url?scp=85027729155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027729155&partnerID=8YFLogxK
U2 - 10.1177/1534735416660383
DO - 10.1177/1534735416660383
M3 - Article
VL - 16
SP - 373
EP - 384
JO - Integrative Cancer Therapies
JF - Integrative Cancer Therapies
SN - 1534-7354
IS - 3
ER -