Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells

Swee Keong Yeap, Nadiah Abu, Nadeem Akthar, Wan Yong Ho, Huynh Ky, Sheau Wei Tan, Noorjahan Banu Alitheen, Tunku Kamarul

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38. However, concurrent activation of antioxidant-related pathways and iron sequestration pathway followed by activation of ER-resident stress proteins clearly indicate that FKB failed to induce apoptosis in HeLa cells via oxidative stress. This effect implies that the protection of HeLa cells by FKB from H2O2-induced cell death is via neutralization of reactive oxygen species.

Original languageEnglish
Pages (from-to)373-384
Number of pages12
JournalIntegrative Cancer Therapies
Volume16
Issue number3
DOIs
Publication statusPublished - 1 Sep 2017

Fingerprint

HeLa Cells
Uterine Cervical Neoplasms
Antioxidants
Gene Expression
Heat-Shock Proteins
Cell Death
Neoplasms
Proteome
Cell Cycle Checkpoints
flavokawain B
Reactive Oxygen Species
Oxidative Stress
Iron
Apoptosis
Cell Line
Messenger RNA

Keywords

  • antioxidant
  • cervical cancer
  • flavokawain B
  • HeLa
  • iron sequestration

ASJC Scopus subject areas

  • Oncology
  • Complementary and alternative medicine

Cite this

Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells. / Yeap, Swee Keong; Abu, Nadiah; Akthar, Nadeem; Ho, Wan Yong; Ky, Huynh; Tan, Sheau Wei; Alitheen, Noorjahan Banu; Kamarul, Tunku.

In: Integrative Cancer Therapies, Vol. 16, No. 3, 01.09.2017, p. 373-384.

Research output: Contribution to journalArticle

Yeap, Swee Keong ; Abu, Nadiah ; Akthar, Nadeem ; Ho, Wan Yong ; Ky, Huynh ; Tan, Sheau Wei ; Alitheen, Noorjahan Banu ; Kamarul, Tunku. / Gene Expression Analysis Reveals the Concurrent Activation of Proapoptotic and Antioxidant-Defensive Mechanisms in Flavokawain B-Treated Cervical Cancer HeLa Cells. In: Integrative Cancer Therapies. 2017 ; Vol. 16, No. 3. pp. 373-384.
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