Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa

James W. Ajioka, John C. Boothroyd, Brian P. Brunk, Adrian Hehl, Ledeana Hillier, Ian D. Manger, Marco Marra, G. Christian Overton, David S. Roos, Kiew Lian Wan, Robert Waterston, L. David Sibley

Research output: Contribution to journalArticle

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Abstract

To accelerate gene discovery and facilitate genetic mapping in the protozoan parasite Toxoplasma gondii, we have generated > 7000 new ESTs from the 5' ends of randomly selected tachyzoite cDNAs. Comparison of the ESTs with the existing gene databases identified possible functions for more than 500 new T. gondii genes by virtue of sequence motifs shared with conserved protein families, including factors involved in transcription, translation, protein secretion, signal transduction, cytoskeleton organization, and metabolism, Despite this success in identifying new genes, more than 50% of the ESTs correspond to genes of unknown function, reflecting the divergent evolutionary status of this parasite. A newly recognized class of genes was identified based on its similarity to sequences known only from other members of the same phylum, therefore identifying sequences that are apparently restricted to the Apicomplexa. Such genes may underlie pathways common to this group of medically important parasites, therefore identifying potential targets for intervention.

Original languageEnglish
Pages (from-to)18-28
Number of pages11
JournalGenome Research
Volume8
Issue number1
Publication statusPublished - Jan 1998

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Apicomplexa
Expressed Sequence Tags
Toxoplasma
Genetic Association Studies
Genes
Parasites
Protein Biosynthesis
Cytoskeleton
Signal Transduction
Complementary DNA
Databases

ASJC Scopus subject areas

  • Genetics

Cite this

Ajioka, J. W., Boothroyd, J. C., Brunk, B. P., Hehl, A., Hillier, L., Manger, I. D., ... Sibley, L. D. (1998). Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa. Genome Research, 8(1), 18-28.

Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa. / Ajioka, James W.; Boothroyd, John C.; Brunk, Brian P.; Hehl, Adrian; Hillier, Ledeana; Manger, Ian D.; Marra, Marco; Overton, G. Christian; Roos, David S.; Wan, Kiew Lian; Waterston, Robert; Sibley, L. David.

In: Genome Research, Vol. 8, No. 1, 01.1998, p. 18-28.

Research output: Contribution to journalArticle

Ajioka, JW, Boothroyd, JC, Brunk, BP, Hehl, A, Hillier, L, Manger, ID, Marra, M, Overton, GC, Roos, DS, Wan, KL, Waterston, R & Sibley, LD 1998, 'Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa', Genome Research, vol. 8, no. 1, pp. 18-28.
Ajioka JW, Boothroyd JC, Brunk BP, Hehl A, Hillier L, Manger ID et al. Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa. Genome Research. 1998 Jan;8(1):18-28.
Ajioka, James W. ; Boothroyd, John C. ; Brunk, Brian P. ; Hehl, Adrian ; Hillier, Ledeana ; Manger, Ian D. ; Marra, Marco ; Overton, G. Christian ; Roos, David S. ; Wan, Kiew Lian ; Waterston, Robert ; Sibley, L. David. / Gene discovery by EST sequencing in Toxoplasma gondii reveals sequences restricted to the apicomplexa. In: Genome Research. 1998 ; Vol. 8, No. 1. pp. 18-28.
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