Gene cloning and characterisation of a new glycosyl hydrolase family 3 β-glucosidase from Aspergillus terreus SUK-1

Nik Marzuki Sidik, Abdul Hani Abdul Manan, Roslina Mat Yazid, Shaiful Adzni Sharifuddin

Research output: Contribution to journalArticle

Abstract

A reverse transcriptase-polymerase chain reaction was used to isolate a gene for β- glucosidase from fungi Aspergillus terreus SUK-1. The DNA consisted of 2586 nucleotides coding sequence encoding 861 amino acids for BGat1 protein. Both nucleotide and amino acid sequences possessed high similarity match with reported β-glucosidase genes and proteins such as of Aspergillus spp. and Neosartorya fischeri. The presence of a 19 amino acids leader sequence suggested that BGat1 is an extracellular protein and very conserve with 5 other β-glucosidases tested. Two specific domains, namely GHF3 N terminal domain and GHF3 C terminal domain, were detected in BGat1 and these domains were separated by a spacer of 99 amino acids. This suggests that BGat1 is a member of glycosyl hydrolase superfamily 3 proteins. Expression analysis showed that BGat1 gene was induced as early as 7 hours after fungal incubation and the level of gene expression maintained at almost the same until day 7. BGat1 expression was also detected when A. terreus SUK-1 was grown in the presence of glucose suggesting that the gene was not inhibited by the product of enzymatic reaction.

Original languageEnglish
Pages (from-to)200-205
Number of pages6
JournalAustralian Journal of Basic and Applied Sciences
Volume6
Issue number2
Publication statusPublished - Feb 2012

Fingerprint

Aspergillus terreus
glucosidases
glycosidases
molecular cloning
amino acids
genes
proteins
nucleotides
enzymatic reactions
Aspergillus
amino acid sequences
reverse transcriptase polymerase chain reaction
gene expression
glucose
fungi
DNA

Keywords

  • β-glucosidase
  • Aspergillus terreus SUK-1
  • Cellulase
  • Expression
  • Glucose

ASJC Scopus subject areas

  • General

Cite this

Gene cloning and characterisation of a new glycosyl hydrolase family 3 β-glucosidase from Aspergillus terreus SUK-1. / Sidik, Nik Marzuki; Abdul Manan, Abdul Hani; Yazid, Roslina Mat; Sharifuddin, Shaiful Adzni.

In: Australian Journal of Basic and Applied Sciences, Vol. 6, No. 2, 02.2012, p. 200-205.

Research output: Contribution to journalArticle

Sidik, Nik Marzuki ; Abdul Manan, Abdul Hani ; Yazid, Roslina Mat ; Sharifuddin, Shaiful Adzni. / Gene cloning and characterisation of a new glycosyl hydrolase family 3 β-glucosidase from Aspergillus terreus SUK-1. In: Australian Journal of Basic and Applied Sciences. 2012 ; Vol. 6, No. 2. pp. 200-205.
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