Gelsolin and ceruloplasmin as potential predictive biomarkers for cervical cancer by 2D-DIGE proteomics analysis

Ilambarthi Lokamani, Mee Lee Looi, Siti Aishah Md Ali, Ahmad Zailani Hatta Mohd Dali, Muhammad Azrif Ahmad Annuar, A. Rahman A. Jamal

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study aimed to identify candidate proteins which may serve as potential biological markers for cervical cancer using 2D-DIGE. Serum samples of controls, patients with cervical intraepithelial neoplasia grade 3 (CIN 3), squamous cell carcinoma of early (SCC I and II) and late (SCC III and IV) stage were subjected to 2D-DIGE. Differentially expressed spots were identified by tandem mass spectrometry. Validation of candidate proteins in serum and tissue samples were then performed by ELISA and immunohistochemistry (IHC) analysis respectively. A total of 20 differentially expressed proteins were identified. These proteins were found to play key roles in the apoptosis pathway, complement system, various types of transportation such as hormones, fatty acids, lipid, vitamin E and drug transportation, coagulation cascade, regulation of iron and immunologic response. Based on their functional relevancy to the progression of various cancers, 4 proteins namely the complement factor H, CD5-like antigen, gelsolin and ceruloplasmin were chosen for further validation using ELISA. Biological network analysis showed that ceruloplasmin and gelsolin are closely interacted with the oncogene NF-κb. These two proteins were further validated using the IHC. Gelsolin and ceruloplasmin may serve as potential predictive biomarkers for the progression of high grade lesions.

Original languageEnglish
Pages (from-to)119-129
Number of pages11
JournalPathology and Oncology Research
Volume20
Issue number1
DOIs
Publication statusPublished - 2014

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Two-Dimensional Difference Gel Electrophoresis
Gelsolin
Ceruloplasmin
Uterine Cervical Neoplasms
Proteomics
Biomarkers
Proteins
CD5 Antigens
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Complement Factor H
Cervical Intraepithelial Neoplasia
Tandem Mass Spectrometry
Vitamin E
Oncogenes
Blood Proteins
Squamous Cell Carcinoma
Fatty Acids
Iron
Hormones

Keywords

  • 2D-DIGE
  • Ceruloplasmin
  • Cervical neoplasia
  • Gelsolin
  • Proteomics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Gelsolin and ceruloplasmin as potential predictive biomarkers for cervical cancer by 2D-DIGE proteomics analysis. / Lokamani, Ilambarthi; Looi, Mee Lee; Md Ali, Siti Aishah; Mohd Dali, Ahmad Zailani Hatta; Ahmad Annuar, Muhammad Azrif; A. Jamal, A. Rahman.

In: Pathology and Oncology Research, Vol. 20, No. 1, 2014, p. 119-129.

Research output: Contribution to journalArticle

Lokamani, Ilambarthi ; Looi, Mee Lee ; Md Ali, Siti Aishah ; Mohd Dali, Ahmad Zailani Hatta ; Ahmad Annuar, Muhammad Azrif ; A. Jamal, A. Rahman. / Gelsolin and ceruloplasmin as potential predictive biomarkers for cervical cancer by 2D-DIGE proteomics analysis. In: Pathology and Oncology Research. 2014 ; Vol. 20, No. 1. pp. 119-129.
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