Gelam honey protects against gamma-irradiation damage to antioxidant enzymes in human diploid fibroblasts

Tengku Ahbrizal Farizal Tengku Ahmad, Zakiah Jubri, Nor Fadilah Rajab, Khairuddin Abdul Rahim, Yasmin Anum Mohd Yusof, Suzana Makpol

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent.

Original languageEnglish
Pages (from-to)2200-2211
Number of pages12
JournalMolecules
Volume18
Issue number2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Honey
fibroblasts
antioxidants
Fibroblasts
Diploidy
enzymes
Antioxidants
Irradiation
catalase
damage
irradiation
Enzymes
Catalase
inorganic peroxides
gene expression
Gene expression
Superoxide Dismutase
enzyme activity
glutathione
Enzyme activity

Keywords

  • Antioxidant enzymes
  • Gamma-irradiation damage
  • Gelam honey
  • Human diploid fibroblasts

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Gelam honey protects against gamma-irradiation damage to antioxidant enzymes in human diploid fibroblasts. / Ahmad, Tengku Ahbrizal Farizal Tengku; Jubri, Zakiah; Rajab, Nor Fadilah; Rahim, Khairuddin Abdul; Mohd Yusof, Yasmin Anum; Makpol, Suzana.

In: Molecules, Vol. 18, No. 2, 02.2013, p. 2200-2211.

Research output: Contribution to journalArticle

Ahmad, Tengku Ahbrizal Farizal Tengku ; Jubri, Zakiah ; Rajab, Nor Fadilah ; Rahim, Khairuddin Abdul ; Mohd Yusof, Yasmin Anum ; Makpol, Suzana. / Gelam honey protects against gamma-irradiation damage to antioxidant enzymes in human diploid fibroblasts. In: Molecules. 2013 ; Vol. 18, No. 2. pp. 2200-2211.
@article{74e9bad1839c4e77815d23877f1ddee8,
title = "Gelam honey protects against gamma-irradiation damage to antioxidant enzymes in human diploid fibroblasts",
abstract = "The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent.",
keywords = "Antioxidant enzymes, Gamma-irradiation damage, Gelam honey, Human diploid fibroblasts",
author = "Ahmad, {Tengku Ahbrizal Farizal Tengku} and Zakiah Jubri and Rajab, {Nor Fadilah} and Rahim, {Khairuddin Abdul} and {Mohd Yusof}, {Yasmin Anum} and Suzana Makpol",
year = "2013",
month = "2",
doi = "10.3390/molecules18022200",
language = "English",
volume = "18",
pages = "2200--2211",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - Gelam honey protects against gamma-irradiation damage to antioxidant enzymes in human diploid fibroblasts

AU - Ahmad, Tengku Ahbrizal Farizal Tengku

AU - Jubri, Zakiah

AU - Rajab, Nor Fadilah

AU - Rahim, Khairuddin Abdul

AU - Mohd Yusof, Yasmin Anum

AU - Makpol, Suzana

PY - 2013/2

Y1 - 2013/2

N2 - The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent.

AB - The present study was designed to determine the radioprotective effects of Malaysian Gelam honey on gene expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to gamma-irradiation. Six groups of HDFs were studied: untreated control, irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs treated with Gelam honey alone showed up-regulation of all genes studied. Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with gamma-irradiation and increased when cells were treated with Gelam honey (p < 0.05). Furthermore, of the three different stages of study treatment, pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes expression and increased the activity of SOD and CAT. As a conclusion, Gelam honey modulates the expression of antioxidant enzymes at gene and protein levels in irradiated HDFs indicating its potential as a radioprotectant agent.

KW - Antioxidant enzymes

KW - Gamma-irradiation damage

KW - Gelam honey

KW - Human diploid fibroblasts

UR - http://www.scopus.com/inward/record.url?scp=84874624933&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874624933&partnerID=8YFLogxK

U2 - 10.3390/molecules18022200

DO - 10.3390/molecules18022200

M3 - Article

VL - 18

SP - 2200

EP - 2211

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 2

ER -