Gelam honey and ginger Potentiate the anti cancer effect of 5-FU against HCT 116 colorectal cancer cells

Luqman Hakim, Ekram Alias, Suzana Makpol, Wan Zurinah Wan Ngah, Nor Azian Morad, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The development of chemopreventive approaches using a concoction of phytochemicals is potentially viable for combating many types of cancer including colon carcinogenesis. This study evaluated the anti-proliferative effects of ginger and Gelam honey and its efficacy in enhancing the anti-cancer effects of 5-FU (5-fluorouracil) against a colorectal cancer cell line, HCT 116. Cell viability was measured via MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphenyl)-2H-tetrazolium) assay showing ginger inhibiting the growth of HCT 116 cells more potently (IC50 of 3mg/mL) in comparison to Gelam honey (IC50 of 75mg/mL). Combined treatment of the two compounds (3mg/mL ginger+75mg/mL Gelam honey) synergistically lowered the IC50 of Gelam honey to 22mg/mL. Combination with 35 mg/mL Gelam honey markedly enhanced 5-FU inhibiting effects on the growth of HCT 116 cells. Subsequent analysis on the induction of cellular apoptosis suggested that individual treatment of ginger and Gelam honey produced higher apoptosis than 5-FU alone. In addition, treatment with the combination of two natural compounds increased the apoptotic rate of HCT 116 cells dose-dependently while treatment of either ginger or Gelam honey combined with 5-FU only showed modest changes. Combination index analysis showed the combination effect of both natural compounds to be synergistic in their inhibitory action against HCT 116 colon cancer cells (CI 0.96 < 1). In conclusion, combined treatment of Gelam honey and ginger extract could potentially enhance the chemotherapeutic effect of 5-FU against colorectal cancer.

Original languageEnglish
Pages (from-to)4651-4657
Number of pages7
JournalAsian Pacific Journal of Cancer Prevention
Volume15
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

Ginger
Honey
Fluorouracil
Colorectal Neoplasms
HCT116 Cells
Neoplasms
Inhibitory Concentration 50
Colonic Neoplasms
Apoptosis
Phytochemicals
Growth
Cell Survival
Carcinogenesis
Cell Line

Keywords

  • 5-FU
  • Anti-proliferation
  • Apoptosis
  • Colon cancer
  • Gelam honey
  • Ginger
  • HCT 116 cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology

Cite this

Gelam honey and ginger Potentiate the anti cancer effect of 5-FU against HCT 116 colorectal cancer cells. / Hakim, Luqman; Alias, Ekram; Makpol, Suzana; Ngah, Wan Zurinah Wan; Morad, Nor Azian; Mohd Yusof, Yasmin Anum.

In: Asian Pacific Journal of Cancer Prevention, Vol. 15, No. 11, 2014, p. 4651-4657.

Research output: Contribution to journalArticle

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