Gelam and nenas honeys inhibit proliferation of HT 29 colon cancer cells by inducing DNA damage and apoptosis while suppressing inflammation

Christinal Teh Pey Wen, Saba Zuhair Hussein, Shailah Abdullah, Norwahidah Abdul Karim, Suzana Makpol, Yasmin Anum Mohd Yusof

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29 Citations (Scopus)

Abstract

Gelam and Nenas monofloral honeys were investigated in this study for their chemopreventive effects against HT 29 colon cancer cells. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolim) assays showed more effective inhibition of colon cancer cells proliferation by Gelam honey with IC50 values of 39.0 mg/ml and 85.5 mg/ml respectively after 24 hours of treatment. Alkali comet assays revealed both honeys increased DNA damage significantly in a dose dependent manner. In addition, annexin V-FITC/PI flow cytometry demonstrated that at IC50 concentrations and above, both Gelam and Nenas honeys induced apoptosis significantlyat values higher than for necrosis (p<0.05). Measurement of prostaglandin E2 (PGE2) confirmed that Gelam and Nenas honeys reduced its production in H2O2 inflammation-induced colon cancer cells. In conclusion, our study indicated and confirmed that both Gelam and Nenas honeys are capable of suppressing the growth of HT 29 colon cancer cells by inducing apoptosis and suppressing inflammation.

Original languageEnglish
Pages (from-to)1605-1610
Number of pages6
JournalAsian Pacific Journal of Cancer Prevention
Volume13
Issue number4
DOIs
Publication statusPublished - 2012

Fingerprint

Honey
Colonic Neoplasms
DNA Damage
Apoptosis
Inflammation
Inhibitory Concentration 50
Comet Assay
Fluorescein-5-isothiocyanate
Annexin A5
Alkalies
Dinoprostone
Flow Cytometry
Necrosis
Cell Proliferation
Growth

Keywords

  • Anti
  • Antitumor
  • Apoptosis
  • DNA damage
  • Gelam honey
  • HT29 cells
  • Inflammatory
  • Nenas honey

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology

Cite this

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title = "Gelam and nenas honeys inhibit proliferation of HT 29 colon cancer cells by inducing DNA damage and apoptosis while suppressing inflammation",
abstract = "Gelam and Nenas monofloral honeys were investigated in this study for their chemopreventive effects against HT 29 colon cancer cells. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolim) assays showed more effective inhibition of colon cancer cells proliferation by Gelam honey with IC50 values of 39.0 mg/ml and 85.5 mg/ml respectively after 24 hours of treatment. Alkali comet assays revealed both honeys increased DNA damage significantly in a dose dependent manner. In addition, annexin V-FITC/PI flow cytometry demonstrated that at IC50 concentrations and above, both Gelam and Nenas honeys induced apoptosis significantlyat values higher than for necrosis (p<0.05). Measurement of prostaglandin E2 (PGE2) confirmed that Gelam and Nenas honeys reduced its production in H2O2 inflammation-induced colon cancer cells. In conclusion, our study indicated and confirmed that both Gelam and Nenas honeys are capable of suppressing the growth of HT 29 colon cancer cells by inducing apoptosis and suppressing inflammation.",
keywords = "Anti, Antitumor, Apoptosis, DNA damage, Gelam honey, HT29 cells, Inflammatory, Nenas honey",
author = "Wen, {Christinal Teh Pey} and Hussein, {Saba Zuhair} and Shailah Abdullah and {Abdul Karim}, Norwahidah and Suzana Makpol and {Mohd Yusof}, {Yasmin Anum}",
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T1 - Gelam and nenas honeys inhibit proliferation of HT 29 colon cancer cells by inducing DNA damage and apoptosis while suppressing inflammation

AU - Wen, Christinal Teh Pey

AU - Hussein, Saba Zuhair

AU - Abdullah, Shailah

AU - Abdul Karim, Norwahidah

AU - Makpol, Suzana

AU - Mohd Yusof, Yasmin Anum

PY - 2012

Y1 - 2012

N2 - Gelam and Nenas monofloral honeys were investigated in this study for their chemopreventive effects against HT 29 colon cancer cells. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolim) assays showed more effective inhibition of colon cancer cells proliferation by Gelam honey with IC50 values of 39.0 mg/ml and 85.5 mg/ml respectively after 24 hours of treatment. Alkali comet assays revealed both honeys increased DNA damage significantly in a dose dependent manner. In addition, annexin V-FITC/PI flow cytometry demonstrated that at IC50 concentrations and above, both Gelam and Nenas honeys induced apoptosis significantlyat values higher than for necrosis (p<0.05). Measurement of prostaglandin E2 (PGE2) confirmed that Gelam and Nenas honeys reduced its production in H2O2 inflammation-induced colon cancer cells. In conclusion, our study indicated and confirmed that both Gelam and Nenas honeys are capable of suppressing the growth of HT 29 colon cancer cells by inducing apoptosis and suppressing inflammation.

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KW - Inflammatory

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