GAPDH as housekeeping gene for human skin fibroblast senescent model.

A. Zainuddin, Suzana Makpol, Chua Kien Hui, N. Abdul Rahim, Yasmin Anum Mohd Yusof, W. Z Wan Ngah

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Validation of housekeeping gene is important for accurate quantitation of RNA in real time RT-PCR technique. The purpose of this study was to determine the validity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a housekeeping gene for quantitative real time RT-PCR assessment in human skin fibroblast senescent model. The cells were divided into different treatment groups; young (passage 4), senescent (passage 30), treatment with H2O2 and treatment with A-tocotrienol prior to H2O2 treatment. Our results showed that the expression level of GAPDH was constant with different treatment groups. Therefore, we concluded that GAPDH was suitable to be used as housekeeping gene in human skin fibroblast senescent model.

Original languageEnglish
Pages (from-to)73-74
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Glyceraldehyde-3-Phosphate Dehydrogenases
Essential Genes
Fibroblasts
Skin
Real-Time Polymerase Chain Reaction
Tocotrienols
RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Zainuddin, A., Makpol, S., Kien Hui, C., Abdul Rahim, N., Mohd Yusof, Y. A., & Ngah, W. Z. W. (2008). GAPDH as housekeeping gene for human skin fibroblast senescent model. The Medical journal of Malaysia, 63 Suppl A, 73-74.

GAPDH as housekeeping gene for human skin fibroblast senescent model. / Zainuddin, A.; Makpol, Suzana; Kien Hui, Chua; Abdul Rahim, N.; Mohd Yusof, Yasmin Anum; Ngah, W. Z Wan.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 73-74.

Research output: Contribution to journalArticle

Zainuddin, A, Makpol, S, Kien Hui, C, Abdul Rahim, N, Mohd Yusof, YA & Ngah, WZW 2008, 'GAPDH as housekeeping gene for human skin fibroblast senescent model.', The Medical journal of Malaysia, vol. 63 Suppl A, pp. 73-74.
Zainuddin A, Makpol S, Kien Hui C, Abdul Rahim N, Mohd Yusof YA, Ngah WZW. GAPDH as housekeeping gene for human skin fibroblast senescent model. The Medical journal of Malaysia. 2008 Jul;63 Suppl A:73-74.
Zainuddin, A. ; Makpol, Suzana ; Kien Hui, Chua ; Abdul Rahim, N. ; Mohd Yusof, Yasmin Anum ; Ngah, W. Z Wan. / GAPDH as housekeeping gene for human skin fibroblast senescent model. In: The Medical journal of Malaysia. 2008 ; Vol. 63 Suppl A. pp. 73-74.
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