Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line

Farahani Abdul Rahman Sazli, Zakiah Jubri, Mariati Abdul Rahman, Saiful Anuar Karsani, Abdul Gapor Md Top, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To determine the antiproliferative effect of gamma-tocotrienol (GTT) treatment on differential protein expression in HepG2 cells. Methods: HepG2 cells were treated with 70 μM GTT for 48 hours and differentially expressed protein spots were determined by two-dimensional electrophoresis (2DE), identified by MALDI-TOF mass spectrometer (MS) and validated by quantitative real-time polymerase chain reaction (qRT-PCR). Results: GTT treatment on HepG2 cells showed a total of five differentially expressed proteins when compared to their respective untreated cells where three proteins were down-regulated and two proteins were up-regulated. One of these upregulated proteins was identified as peroxiredoxin-4 (Prx4). Validation by qRT-PCR however showed decreased expression of Prx4 mRNA in HepG2 cells following GTT treatment. Conclusions: GTT might directly influence the expression dynamics of peroxiredoxin-4 to control proliferation in liver cancer.

Original languageEnglish
Article number64
JournalBMC Complementary and Alternative Medicine
Volume15
Issue number1
DOIs
Publication statusPublished - 13 Mar 2015

Fingerprint

Peroxiredoxins
Liver Neoplasms
Hep G2 Cells
Cell Line
Proteins
Real-Time Polymerase Chain Reaction
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis
plastochromanol 8
Messenger RNA

Keywords

  • Gamma-tocotrienol
  • HepG2 cells
  • Peroxiredoxin-4
  • Proteomics

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line. / Abdul Rahman Sazli, Farahani; Jubri, Zakiah; Abdul Rahman, Mariati; Karsani, Saiful Anuar; Md Top, Abdul Gapor; Wan Ngah, Wan Zurinah.

In: BMC Complementary and Alternative Medicine, Vol. 15, No. 1, 64, 13.03.2015.

Research output: Contribution to journalArticle

Abdul Rahman Sazli, Farahani ; Jubri, Zakiah ; Abdul Rahman, Mariati ; Karsani, Saiful Anuar ; Md Top, Abdul Gapor ; Wan Ngah, Wan Zurinah. / Gamma-tocotrienol treatment increased peroxiredoxin-4 expression in HepG2 liver cancer cell line. In: BMC Complementary and Alternative Medicine. 2015 ; Vol. 15, No. 1.
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AU - Karsani, Saiful Anuar

AU - Md Top, Abdul Gapor

AU - Wan Ngah, Wan Zurinah

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