Gamma-tocotrienol modulated gene expression in senescent human diploid fibroblasts as revealed by microarray analysis

Suzana Makpol, Azalina Zainuddin, Chua Kien Hui, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of γ-tocotrienol, a vitamin E isomer, in modulating gene expression in cellular aging of human diploid fibroblasts was studied. Senescent cells at passage 30 were incubated with 70 M of γ-tocotrienol for 24 h. Gene expression patterns were evaluated using Sentrix HumanRef-8 Expression BeadChip from Illumina, analysed using GeneSpring GX10 software, and validated using quantitative RT-PCR. A total of 100 genes were differentially expressed (P < 0.001) by at least 1.5 fold in response to γ-tocotrienol treatment. Amongst the genes were IRAK3, SelS, HSPA5, HERPUD1, DNAJB9, SEPR1, C18orf55, ARF4, RINT1, NXT1, CADPS2, COG6, and GLRX5. Significant gene list was further analysed by Gene Set Enrichment Analysis (GSEA), and the Normalized Enrichment Score (NES) showed that biological processes such as inflammation, protein transport, apoptosis, and cell redox homeostasis were modulated in senescent fibroblasts treated with γ-tocotrienol. These findings revealed that γ-tocotrienol may prevent cellular aging of human diploid fibroblasts by modulating gene expression.

Original languageEnglish
Article number454328
JournalOxidative Medicine and Cellular Longevity
DOIs
Publication statusPublished - 2013

Fingerprint

Tocotrienols
Fibroblasts
Microarray Analysis
Microarrays
Diploidy
Gene expression
Gene Expression
Genes
Cell Aging
Aging of materials
Biological Phenomena
Protein Transport
Vitamin E
Isomers
Oxidation-Reduction
Homeostasis
Software
plastochromanol 8
Apoptosis
Inflammation

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry

Cite this

Gamma-tocotrienol modulated gene expression in senescent human diploid fibroblasts as revealed by microarray analysis. / Makpol, Suzana; Zainuddin, Azalina; Kien Hui, Chua; Mohd Yusof, Yasmin Anum; Ngah, Wan Zurinah Wan.

In: Oxidative Medicine and Cellular Longevity, 2013.

Research output: Contribution to journalArticle

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