Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing

Sarada Idris, Nur Hidayah Azeman, Noor Azwen Noor Azmy, Chantara Thevy Ratnam, Mohd Adzir Mahdi, Ahmad Ashrif A Bakar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tapered optical fiber coated with pyrrole/poly(vinyl alcohol)-glucose oxidase (Py/PVA-GOx) for glucose biosensing was successfully fabricated by radiation immobilization of GOx onto polymeric surfaces. Polymerization of pyrrole and PVA crosslinking was carried out by means of gamma irradiation. The nature of enzyme immobilization was studied by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and atomic force microscopy. The observation of an absorption band at 1650 cm−1 and binding energy formed at 287.5 eV confirm the occurrence of GOx immobilization on the polymer matrix. An increase in film thickness is observed after irradiation, which confirms the entrapment of GOx into the Py/PVA polymer matrix. The peak to valley roughness for the irradiated Py/PVA-GOx reveals the intermolecular interaction between the polymers and enzyme. These characteristics are linked to the enzymatic reaction of the coated optical fiber towards the glucose concentration. The kinetic property of the GOx in the irradiated Py/PVA-GOx coated fiber was studied with a very low value obtained for the Michaelis-Menten constant, which contributes to improved adhesion and immobilization on the coated fiber. The response and sensitivity of the coated optical fiber were recorded as <0.31 μW and 8.7 × 10-3 μWmM-1, respectively. A selectivity study reveals that the irradiated fiber coated with Py/PVA-GOx is highly selective towards glucose.

Original languageEnglish
Pages (from-to)1404-1412
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume273
DOIs
Publication statusPublished - 10 Nov 2018

Fingerprint

Pyrroles
Glucose oxidase
immobilization
Glucose Oxidase
glucose
Glucose
Optical fibers
Alcohols
optical fibers
pyrroles
oxidase
Polymer matrix
alcohols
Fibers
Irradiation
Enzyme immobilization
Enzyme kinetics
fibers
Binding energy
enzymes

Keywords

  • Gamma irradiation
  • Glucose sensing
  • Optical fiber biosensor
  • Poly(vinyl alcohol)
  • Pyrrole

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing. / Idris, Sarada; Azeman, Nur Hidayah; Noor Azmy, Noor Azwen; Ratnam, Chantara Thevy; Mahdi, Mohd Adzir; A Bakar, Ahmad Ashrif.

In: Sensors and Actuators, B: Chemical, Vol. 273, 10.11.2018, p. 1404-1412.

Research output: Contribution to journalArticle

Idris, Sarada ; Azeman, Nur Hidayah ; Noor Azmy, Noor Azwen ; Ratnam, Chantara Thevy ; Mahdi, Mohd Adzir ; A Bakar, Ahmad Ashrif. / Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing. In: Sensors and Actuators, B: Chemical. 2018 ; Vol. 273. pp. 1404-1412.
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abstract = "Tapered optical fiber coated with pyrrole/poly(vinyl alcohol)-glucose oxidase (Py/PVA-GOx) for glucose biosensing was successfully fabricated by radiation immobilization of GOx onto polymeric surfaces. Polymerization of pyrrole and PVA crosslinking was carried out by means of gamma irradiation. The nature of enzyme immobilization was studied by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and atomic force microscopy. The observation of an absorption band at 1650 cm−1 and binding energy formed at 287.5 eV confirm the occurrence of GOx immobilization on the polymer matrix. An increase in film thickness is observed after irradiation, which confirms the entrapment of GOx into the Py/PVA polymer matrix. The peak to valley roughness for the irradiated Py/PVA-GOx reveals the intermolecular interaction between the polymers and enzyme. These characteristics are linked to the enzymatic reaction of the coated optical fiber towards the glucose concentration. The kinetic property of the GOx in the irradiated Py/PVA-GOx coated fiber was studied with a very low value obtained for the Michaelis-Menten constant, which contributes to improved adhesion and immobilization on the coated fiber. The response and sensitivity of the coated optical fiber were recorded as <0.31 μW and 8.7 × 10-3 μWmM-1, respectively. A selectivity study reveals that the irradiated fiber coated with Py/PVA-GOx is highly selective towards glucose.",
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