Galactose-binding lectin from the seeds of champedak (Artocarpus integer): Sequences of its subunits and interactions with human serum O-glycosylated glycoproteins

Mariati Abdul Rahman, Saiful Anuar Karsani, Iekhsan Othman, Puteri Shafinaz Abdul Rahman, Onn Haji Hashim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Our group has previously reported the isolation, partial characterisation, and application of a Galβ1-3GalNAc- and IgA1-reactive lectin from the seeds of champedak (Artocarpus integer). In the present study, we have subjected the purified lectin to reverse-phase high performance liquid chromatography and sequenced its subunits. Determination of the N-terminal sequence of the first 47 residues of the large subunit demonstrated at least 95% homology to the N-terminal sequence of the α chains of a few other galactose-binding Artocarpus lectins. The two smaller subunits of the lectin, each comprised of 21 amino acid residues, demonstrated minor sequence variability. Their sequences were generally comparable to the β chains of the other galactose-binding Artocarpus lectins. When used to probe human serum glycopeptides that were separated by two-dimensional gel electrophoresis, the lectin demonstrated strong apparent interactions with glycopeptides of IgA1, hemopexin, α2-HS glycoprotein, α1-antichymotrypsin, and a few unknown glycoproteins. Immobilisation of the lectin to Sepharose generated an affinity column that may be used to isolate the O-glycosylated serum glycoproteins.

Original languageEnglish
Pages (from-to)1007-1013
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume295
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Artocarpus
Galectins
Lectins
Seed
Seeds
Glycoproteins
Serum
Glycopeptides
Immunoglobulin A
Hemopexin
Galactose
Electrophoresis, Gel, Two-Dimensional
Reverse-Phase Chromatography
Immobilization
Sepharose
High performance liquid chromatography
High Pressure Liquid Chromatography
Electrophoresis
Amino Acids
Gels

Keywords

  • Artocarpus integer
  • Lectin
  • O-Glycan
  • Sequence
  • Serum glycoproteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Galactose-binding lectin from the seeds of champedak (Artocarpus integer) : Sequences of its subunits and interactions with human serum O-glycosylated glycoproteins. / Abdul Rahman, Mariati; Anuar Karsani, Saiful; Othman, Iekhsan; Shafinaz Abdul Rahman, Puteri; Haji Hashim, Onn.

In: Biochemical and Biophysical Research Communications, Vol. 295, No. 4, 2002, p. 1007-1013.

Research output: Contribution to journalArticle

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