Future applications of apricot (Prunus Armeniaca Kaisa) ß galactosidase in dairy industry

Shakeel Ahmed Ansari, Rukhsana Satar, Syed Kashif Zaidi, Mohd Jahir Khan, Muhammad Imran Naseer, Mohammed H. Al-Qahtani, Mohamad Yusof Maskat

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study demonstrates the immobilization of β galactosidase from apricots (Prunus armeniaca kaisa) on an inexpensive concanavalin A layered cellulose-alginate hybrid gel. Immobilized β galactosidase retained 78% of the initial activity after crosslinking by glutaraldehyde. It exhibited greater fraction of activity at both acidic and basic pH, and showed broad spectrum temperature optimum as compared to free enzyme. Moreover, immobilized enzyme exhibited higher thermal stability at 60°C and retained 80% of the original enzyme activity in presence of 3% galactose. The crosslinked immobilized enzyme showed improved hydrolysis of lactose from milk and whey in batch processes at 50°C as well as in continuous reactors operated at fl ow rate of 20 mL/h and 30 mL/h even after one month. Moreover, crosslinked adsorbed β galactosidase retained 76% activity even after its sixth repeated use, thereby promoting its use for lactose hydrolysis in various dairy products even for longer durations.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalPolish Journal of Chemical Technology
Volume16
Issue number3
DOIs
Publication statusPublished - 1 Sep 2014

Fingerprint

Dairying
Galactosidases
Dairies
Immobilized Enzymes
Enzymes
Lactose
Hydrolysis
Dairy products
Industry
Dairy Products
Alginate
Enzyme activity
Glutaral
Concanavalin A
Galactose
Cellulose
Immobilization
Crosslinking
Milk
Thermodynamic stability

Keywords

  • Apricots
  • dairy industries
  • lactose hydrolysis
  • β galactosidase

ASJC Scopus subject areas

  • Biotechnology
  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Future applications of apricot (Prunus Armeniaca Kaisa) ß galactosidase in dairy industry. / Ansari, Shakeel Ahmed; Satar, Rukhsana; Zaidi, Syed Kashif; Khan, Mohd Jahir; Naseer, Muhammad Imran; Al-Qahtani, Mohammed H.; Maskat, Mohamad Yusof.

In: Polish Journal of Chemical Technology, Vol. 16, No. 3, 01.09.2014, p. 74-79.

Research output: Contribution to journalArticle

Ansari, Shakeel Ahmed ; Satar, Rukhsana ; Zaidi, Syed Kashif ; Khan, Mohd Jahir ; Naseer, Muhammad Imran ; Al-Qahtani, Mohammed H. ; Maskat, Mohamad Yusof. / Future applications of apricot (Prunus Armeniaca Kaisa) ß galactosidase in dairy industry. In: Polish Journal of Chemical Technology. 2014 ; Vol. 16, No. 3. pp. 74-79.
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