Functions of human cytosolic domain of β-common subunit receptor GM-CSF in FDCP-MIX stem cells

Shahrul Hisham Zainal Ariffin, Rohaya Megat Abdul Wahab, Zaidah Zainal Ariffin, A. D. Whetton

Research output: Contribution to journalArticle

Abstract

The mechanisms of cytokines or growth factors mediate the regulation of cell survival, proliferation and differentiation into mature cells remain unclear. The cytokine interleukin-3 and Granulocyte-macrophage Colony Stimulating Factor (GM-CSF) play important roles in the survival, growth and development of haemopoeitic stem cells. Activation of human IL-3 or GM-CSF receptor ectopically expressed in polyclonal murine FDCP-Mix multipotent cell will stimulate self-renewal or myeloid differentiation respectively. Each of the GM-CSF and IL-3 receptors comprises a unique ligand-specific α subunit and a common β subunit (βc). The common βc subunit has a relatively large cytosolic domain compared to α subunit thus considered to play a pivotal role in receptor's signal transduction. In these studies, the chimeric receptor composed of the extracellular domain of GM-CSF and cytosolic domain of βc was generated, i.e., hGM-CSFR/βc. The respective receptor was expressed in the FDCP-Mix stem cells, showed that the receptor was capable in promoting maintenance of primitive phenotype in the absence of α subunit. This result showed that the βc subunit alone could contribute signals principally for proliferation and self-renewal.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalBiotechnology
Volume6
Issue number2
Publication statusPublished - 2007

Fingerprint

Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Stem cells
Stem Cells
Interleukin-3
Interleukin-3 Receptors
Cytokines
Signal transduction
Cell proliferation
Growth and Development
Cell Differentiation
Signal Transduction
Cell Survival
Intercellular Signaling Peptides and Proteins
Chemical activation
Maintenance
Cell Proliferation
Ligands
Phenotype

Keywords

  • Cell's signals
  • Cytosolic domain
  • Haemopoietic stem cells
  • Human βc subunit

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials

Cite this

Functions of human cytosolic domain of β-common subunit receptor GM-CSF in FDCP-MIX stem cells. / Zainal Ariffin, Shahrul Hisham; Megat Abdul Wahab, Rohaya; Ariffin, Zaidah Zainal; Whetton, A. D.

In: Biotechnology, Vol. 6, No. 2, 2007, p. 225-231.

Research output: Contribution to journalArticle

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